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Method for preparing irradiation crosslinking heterogeneous skin acellular matrix and products produced thereby

An acellular matrix, xenogeneic technology, applied in medical science, prosthesis, etc., can solve the problems of incomplete decellularization, damage to the flexibility and randomness of the scaffold structure, and inconvenience.

Inactive Publication Date: 2009-07-01
郭翔
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: 1. Such a low concentration of trypsin is not completely decellularized at this temperature; 2. Repeated freezing and thawing will damage the softness and randomness of the scaffold structure
Additionally, using 60 Co is a radioactive source. Even if the source is 500,000 curies, it will take more than 15 hours to meet the sterilization requirements for biological skin preparations with a radiation dose of 6-30KGry / h, which will cause great harm in actual production. inconvenient

Method used

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  • Method for preparing irradiation crosslinking heterogeneous skin acellular matrix and products produced thereby
  • Method for preparing irradiation crosslinking heterogeneous skin acellular matrix and products produced thereby
  • Method for preparing irradiation crosslinking heterogeneous skin acellular matrix and products produced thereby

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Effect test

Embodiment 1

[0043] The patient was a male, 49 years old, with flame burns, the total burn area was 79%, and deep burns on the left leg. The hog dermis was transplanted with trolley cells, and the reticular skin was evenly spread, and healed after 3 to 4 weeks. After half a year of treatment, the wound showed no scars.

Embodiment 2

[0045]The patient was a 67-year-old female with a history of diabetes for 10 years. Three months after the burn, the wound was infected by staphylococcus. After autologous microskin was transplanted and covered with porcine dermis acellular scaffold, the wound healed within 4 weeks.

[0046] Clinical Trials:

[0047] Adopting the method of decellularized allogeneic dermis or irradiated cross-linked heterogeneous skin acellular matrix made by the method of the present invention plus autologous blade-thick skin transplantation, repaired 119 cases of various wounds, compared the skin graft survival rates of various wounds, observed The relationship between the application of different parts of skin covering, decellularized allogenic dermis, or the acellular matrix of irradiated cross-linked heterogeneous skin prepared by the method of the present invention and the survival of skin grafts, and some cases were observed histologically and followed up. Results: The survival rates of...

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Abstract

The present invention belongs to the field of animal leather product for medicine. The method for preparing radiation sterilized allogenous acellular dermal matrix mainly comprises the following steps: preparing skin; cutting skin with machine for separating corium and epidermis and removing cell: first removing: cleaning with pure water after immersing the skin in 10-20% of parenzyme for 30-50 minutes in 36-40 DEG C; and second removing: alternately immersing the skin piece which has a water content of 10-30% after water logging in 10-20% of deoxyribonuclease and 10-301051632074f ribalgilase for 2-4 times in 36-40 DEG C, and immersing the skin piece in 0.5-1.5% of deoxyribonuclease or ribalgilase for 5-30 minutes in 36-40 DEG C for removing the substance which can initiate the recognition reaction of host cell in the corium; immersing the processed material in common fixing agent for 50-120 minutes; and radiating for sterilization, namely executing gamma-ray radiation sterilization through electron accelerator or Co-60.

Description

technical field [0001] The invention belongs to the field of medical biological leather products, in particular to the field of biological skin decellularized mesh support. Background technique [0002] Biomaterials play an important role in the treatment of patients with extensive burns. In recent years, skin experts at home and abroad have been looking for good wound repair covering materials. For the purpose of eliminating antigenicity and rejection, making heterogeneous skins for cryopreservation and permanent skin wound replacement materials is an urgently needed new technology. In order to improve the curative effect of rescuing patients with large area burns, it has broad prospects. [0003] A cross-linked porcine decellularized skin sheet is disclosed in Chinese patent CN1266716A. Use 0.25% trypsin at 4°C, soak for 16-24 hours to decellularize, and then peel off the epidermis. The reason for taking such a long time is that the decellularization level of trypsin is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60
Inventor 郭翔
Owner 郭翔
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