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Hair root of salvia miltiorrhiza containing various compounds of high content and culturing technique thereof

A hairy root and phenolic acid technology, applied in the field of hairy root SmHr1 of Salvia miltiorrhiza and its culture field, can solve the problems of increasing extraction cost, occupying cultivated land and the like, and achieve the effects of increasing growth accumulation, improving purity and reducing production cost.

Inactive Publication Date: 2009-07-29
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If we only rely on large-scale cultivation of Salvia miltiorrhiza to extract salvianolic acid compounds, it will not only occupy arable land, but also increase the cost of extraction.

Method used

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  • Hair root of salvia miltiorrhiza containing various compounds of high content and culturing technique thereof
  • Hair root of salvia miltiorrhiza containing various compounds of high content and culturing technique thereof
  • Hair root of salvia miltiorrhiza containing various compounds of high content and culturing technique thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Composition of YMB liquid medium (g / L)

[0099] Manitol 10

[0100] Yeast Extract 0.4

[0101] NaCl 0.1

[0102] MgSO 4 ·7H 2 O 0.2

[0103] K 2 HPO 4 0.5

[0104] Adjust the pH to 7.2 with NaOH and HCl and autoclave at 121°C.

[0105] Composition of MSOH solid medium (mg / L)

[0106] (1) Major elements

[0107] KNO 3 1,900

[0108] CaCl 2 2H 2 O 440

[0109] MgSO 4 ·7H 2 O 370

[0110] K H 2 PO 4 170

[0111] (2) Trace elements

[0112] KI 0.83

[0113] h 3 BO 3 6.2

[0114] MnSO 4 4H 2 O 22.3

[0115] ZnSO 4 ·7H 2 O 8.6

[0116] Na 2 MoO 4 2H 2 O 0.25

[0117] CuSO 4 ·5H 2 O 0.025

[0118] CoCl 2 ·6H 2 O 0.025

[0119] (3) Fe salt

[0120] Na 2 EDTA·2H 2 O 37.3

[0121] FeSO 4 ·7H 2 O 27.8

[0122] (4) Organic elements

[0123] Inositol 100

[0124] Niacin 0.5

[0125] Pyridoxine hydrochloride 0.5

[0126] Ammonium sulfate hydrochloride 0.4

[0127] Glycine 2.0

[01...

Embodiment 2

[0154] Danshen hairy root obtained

[0155] (1) Preparation of Agrobacterium rhizogenes:

[0156] A single colony of Agrobacterium rhizogenes LBA9402 was picked from the plate, inoculated into YMB liquid medium, and cultured for 2 days at 28° C. and 250 rpm with horizontal gyratory shaking.

[0157] (2) Infection:

[0158] Remove the leaves from the aseptic seedlings, and cut the leaves into leaf discs with medical scissors; dilute the above-mentioned Agrobacterium rhizogenes bacterium liquid cultivated overnight; Bacteria filter paper was used to absorb excess Agrobacterium on the leaf disk, and placed on MSOH solid medium at 25°C for 2 days in the dark.

[0159] (3) Screening of transformants:

[0160] The leaves infected by Agrobacterium rhizogenes were transferred to MSOH solid medium, and cultured in the dark at 25°C; after 1 week, hairy roots grew around the incision of the leaf disc; when the hairy roots grew to about 2cm, cut off and transfer into MSOH solid medium...

Embodiment 3

[0168] Danshen hairy root obtained

[0169] (1) Preparation of Agrobacterium rhizogenes:

[0170] A single colony of Agrobacterium rhizogenes LBA9402 was picked from the plate, inoculated into YMB liquid medium, and cultured for 2 days at 28° C. and 250 rpm with horizontal gyratory shaking.

[0171] (2) Infection:

[0172] Remove the leaves from the aseptic seedlings, and cut the leaves into leaf disks with medical scissors; dilute the Agrobacterium rhizogenes bacterium liquid cultivated overnight by 10 times; put the leaf disks into the diluted Agrobacterium suspension and soak for 5 minutes, no Bacteria filter paper was used to absorb excess Agrobacterium on the leaf disk, and placed on MSOH solid medium at 25°C for 2 days in the dark.

[0173] (3) Screening of transformants:

[0174] Transfer the leaves infected by Agrobacterium rhizogenes to MSOH solid medium, and culture them in the dark at 25°C; after 10 days, hairy roots grow around the incision of the leaf disk; whe...

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Abstract

The invention provides a radix salivae miltiorrhizae hairy root SmHr1 with high-content phenolic compounds and a method for cultivating the same. The radix salivae miltiorrhizae hairy root contains salvianolic acid B, salvianolic acid K and a salvianolic acid and rosmarinci acid compounds; and the content of the radix salivae miltiorrhizae hairy root is higher than that of unileaf sage root. The cultivation method is applied and can remarkably improve the accumulated growth amount of the radix salivae miltiorrhizae hairy root and the content of the salvianolic acid compounds as medical compositions, thereby proving a high-yield strain for large-scale production of the compounds, reducing the production cost and promoting industrialized production.

Description

technical field [0001] The present invention relates to traditional Chinese medicine. In particular, it relates to a Salvia miltiorrhiza hairy root SmHr1 with high content of multiple phenolic compounds and its culture technology. Background technique [0002] Salvia miltiorrhiza Bunge is a commonly used traditional Chinese medicine, and its medicinal part is the root. Salvia miltiorrhiza and traditional Chinese medicine preparations based on danshen are one of the first-choice medicines for clinical treatment of cardiovascular diseases. In addition to being used in decoction pieces, Danshen is also the main raw material of many kinds of Chinese patent medicines such as Compound Danshen Dripping Pills, Compound Danshen Tablets, and Danshen Injection. [0003] The main active ingredients of Danshen include diterpene quinones such as tanshinone and water-soluble phenolic acids such as salvianolic acid. The medicinal value of salvianolic acid compounds has been paid more and...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61K31/216A61P9/00A01H4/00A61K125/00
Inventor 赵淑娟王峥涛胡之璧周吉燕张金家
Owner SHANGHAI UNIV OF T C M
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