Technique for refining L-tryptophan

A tryptophan and process technology, applied in the field of L-tryptophan refining process, can solve the problems of product content, transmittance and specific rotation failing to meet the quality standard requirements, etc.

Inactive Publication Date: 2009-10-28
河南孟成生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the production of L-tryptophan by fermentation method solves the problem of yield, in the process of fermentation production, partially metabolized water-soluble protein and the remain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0010] Example 1

[0011] The crude L-tryptophan is 20 g, and the crude product contains 6 g of L-tryptophan. A mixed solvent of 100 g of water and propanol is used as a solvent to dissolve the crude L-tryptophan. The solvent contains 83.3 g of water and 16.7 g of propanol. After the crude product is completely dissolved, react at 50°C for 100 min. After filtration treatment, a transparent and clear product-containing solution is obtained. The solution is passed through a thin film evaporation device to remove water to obtain wet L-tryptophan. After drying treatment , Get 5.1g L-tryptophan fine product (product yield 85%, L-tryptophan mass percentage 98.5%), according to the conventional method to determine the light transmittance and optical rotation: the light transmittance is 90.5% (relative Value), the optical rotation is -30 degrees, which meets the requirements of quality standards.

Example Embodiment

[0012] Example 2

[0013] 25 g of crude L-tryptophan, the crude product contains log of L-tryptophan, and a mixed solvent of 100 g of water and propanol is used as a solvent to dissolve the crude L-tryptophan, wherein the solvent contains 80 g of water and 20 g of propanol. After the crude product is completely dissolved, react at 65°C for 110 min. After filtration treatment, a transparent and clear product-containing solution is obtained. The solution is passed through a thin film evaporation device to remove water to obtain wet L-tryptophan. After drying treatment , Get 8.8g L-tryptophan fine product (product yield 88%, L-tryptophan mass percentage 98.8%), according to the conventional method to measure its light transmittance and optical rotation, known: light transmittance is 91.5% (relative Value), the optical rotation is -31.3 degrees, which meets the requirements of quality standards.

Example Embodiment

[0014] Example 3

[0015] 30 g of crude L-tryptophan, containing 15 g of L-tryptophan in the crude product, use a mixed solvent of 100 g of water and propanol as a solvent to dissolve the crude L-tryptophan, in which the solvent contains 66.7 g of water and 33.3 g of propanol. After the crude product is completely dissolved, react at 80°C for 120 min. After filtration treatment, a transparent and clear product-containing solution is obtained. The solution is passed through a thin film evaporation device to remove water to obtain wet L-tryptophan. After drying treatment , Get 13.8g L-tryptophan fine product (product yield 92%, L-tryptophan mass percentage 99%), according to the conventional method to measure the light transmittance and optical rotation, we know that the light transmittance is 92% (relative Value), the optical rotation is -32.5 degrees, which meets the requirements of quality standards.

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Abstract

The invention pertains to the technical field of biological engineering and particularly discloses a technique for refining L-tryptophan. The mixed solvent of water and propanol is taken as a dissolvent for dissolving L-tryptophan crude product; after the L-tryptophan crude product is dissolved completely, a reaction is conducted on the solution at the temperature of 50-80 DEG C for 100-120min and a transparent and clean solution containing the product is obtained by filtration, wet L-tryptophan is obtained by removing water from the solution, and then fine L-tryptophan product is obtained by drying the wet L-tryptophan. The technique is simple. In the fine L-tryptophan product obtained by the refining technique, the mass percentage of the L-tryptophan is over 98.5 percent, transmittance is higher or equal to 90 percent (a relative value), optical rotation is -30.0 degrees to -32.5 degrees, all meet requirements for quality standard, and product yield is higher than 85 percent.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a process for refining L-tryptophan. Background technique [0002] L-Tryptophan is used as a medical, feed grade or food grade additive, which has high requirements for product content, light transmittance and specific rotation. Although the production of L-tryptophan by fermentation method solves the problem of yield, in the process of fermentation production, some metabolized water-soluble protein and the remaining protein of raw and auxiliary materials will enter the product, resulting in a decrease in product content and light transmittance. , The specific rotation does not meet the quality standard requirements. Therefore, how to develop a refining process for L-tryptophan so that its product content, light transmittance, and specific rotation all meet the quality standard requirements is undoubtedly necessary and even more urgent. Contents of the inven...

Claims

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Application Information

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IPC IPC(8): C07D209/20
Inventor 孟军虎王进全武涛
Owner 河南孟成生物药业股份有限公司
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