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Distinguishing method of juglanin B in pericarpium juglandis and content measuring method thereof

A technology of juicylin and qinglong, applied in the identification field of jugulin B, can solve the problems such as lack of stable and effective quality control indicators of qinglongyi and its preparations, and achieve the effects of easy extraction and separation, good reproducibility and quality control

Inactive Publication Date: 2010-02-03
HEILONGJIANG UNIV
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide the identification method and content determination method of Juglandin B in Qinglongyi, to solve the problem that the existing Qinglongyi and its preparations lack stable and effective quality control indicators

Method used

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  • Distinguishing method of juglanin B in pericarpium juglandis and content measuring method thereof
  • Distinguishing method of juglanin B in pericarpium juglandis and content measuring method thereof
  • Distinguishing method of juglanin B in pericarpium juglandis and content measuring method thereof

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specific Embodiment approach 1

[0011] Specific embodiment one: the identification method of juglin B in Qinglongyi in the present embodiment is realized by thin layer chromatography (thin layer chromatography, thin Layer Chromatography), specifically is carried out according to the following steps: a, the crude drug of Qinglongyi Pass the powder through a No. 3 sieve (average sieve inner diameter 355 ± 13, industrial sieve mesh 60 holes / inch), then weigh 10 g, add 50 mL volume of ethanol with a purity of 50% to 95%, ultrasonicate for 30 minutes, and filter. Remove the filter residue, then evaporate the filtrate to dryness, add methanol 1mL to the residue obtained after evaporation to dissolve, as the test solution; b, add 1mg juglanin B to every milliliter of methanol to prepare a reference substance solution; c, absorb the test solution respectively 10 μL each of the reference substance solution and the reference substance solution were spotted on the same silica gel G plate, developed with a developing age...

specific Embodiment approach 2

[0012] Embodiment 2: This embodiment differs from Embodiment 1 in that: the volume purity of the ethanol described in step a is 50%. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0013] Embodiment 3: This embodiment differs from Embodiment 1 in that: the volume purity of the ethanol described in step a is 60%-80%. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a distinguishing method of juglanin B in pericarpium juglandis and a content measuring method thereof, relating to a distinguishing method of juglanin B and a content measuringmethod thereof and solving the problem of lacking stable and effective quality control indexes of the existing pericarpium juglandis and preparations thereof. The distinguishing method of the juglanin B is realized through a thin-layer chromatography, and the content measuring method of the juglanin B is carried out by adopting a high-efficiency liquid-phase chromatography. The methods control the quality of the pericarpium juglandis and the preparations thereof by using the juglanin B as a marker component, thereby having rationality and operability. The thin-layer chromatography and the high-efficiency liquid-phase chromatography have simplicity and good repeatability, can effectively control the quality of pericarpium juglandis medicinal materials and preparations thereof so as to realize the stability and the controllability of the pericarpium juglandis medicinal materials and the preparations thereof and have specificity, accuracy and reliability compared with the existing quality standard. The juglanin B can be used as a traditional Chinese medicine reference substance to be widely applied.

Description

technical field [0001] The invention relates to a method for identifying juglandin B and a method for determining its content. Background technique [0002] Qinglongyi is the immature exocarp of Juglans mandshurica MAXIM. and Juglans regia L. (Wu Zhengyi, Zhou Taiyan, Xiao Peigen, etc. Xinhua Compendium of Materia Medica (Volume III). Shanghai: Shanghai Science and Technology Press, 1990); effective treatment of gastric cancer, liver cancer, esophageal cancer, lung cancer and other digestive system tumors. [0003] At present, the quality standards of Qinglongyi and its preparations do not include identification and content determination methods for the chemical components of Qinglongyi, so the quality of medicinal materials and preparations cannot be effectively controlled, and they do not have specificity, accuracy and reliability. Contents of the invention [0004] The object of the present invention is to provide a method for identifying juglandin B in Qinglongyi and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P1/00A61P35/00G01N30/36G01N30/90A61K131/00
Inventor 刘丽娟
Owner HEILONGJIANG UNIV
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