Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Human plasminogen K5 modified by RGD and preparation method and application thereof

A technology of human plasminogen and RGD-K5, applied in the direction of microorganism-based methods, biochemical equipment and methods, medical preparations of non-active ingredients, etc., can solve the high production cost of yeast system, the lack of release methods, and the application High dose and other problems, to achieve the effect of improving anti-tumor effect, good anti-angiogenesis and anti-tumor effect, and reducing side effects

Inactive Publication Date: 2010-02-17
XIAMEN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional K5 has defects such as lack of targeting in the release method, large application dose, high production cost of the yeast system, and potential side effects, which seriously restrict the clinical application of K5.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human plasminogen K5 modified by RGD and preparation method and application thereof
  • Human plasminogen K5 modified by RGD and preparation method and application thereof
  • Human plasminogen K5 modified by RGD and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific implementation examples. It should be understood that these implementation examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0030] 1. Design and construction of pPIC9K-RGD-K5 vector:

[0031] (1) Design of primers: According to the full-length sequence of K5 and the multiple cloning site of expression vector pPIC9K, design and synthesize upstream primers and downstream primers containing RGD motifs (synthesized by Saibaisheng Company), upstream primers: 5'-CG CTC GAG AAAAGA GCATGC GAC TGC CGT GGT GAC TGC TTC TGC GGT GGT GGT GGT GTC CTG CTT CCAGAT GTA G; downstream primer: 3'-GC GAA TTC TAG GCC GCA CAC TGA GGG AC.

[0032] (2) Construction of RGD-K5 sequence: Using the pPIC9K-K5 plasmid as a template, use the above primers to connect RGD to the N-terminus of K5 by PCR, cycle parameters: 94°C 2min, (94°C 45Sec, 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses to a human plasminogen K5 modified by RGD and a preparation method and application thereof and relates to a human plasminogen and provides a human plasminogen K5 modified by anantineoplastic protein-RGD, a preparation method for the human plasminogen K5 and application of the human plasminogen K5 in the antineoplaston. The amino acid sequence of the human plasminogen K5 isACDCRGDCFCGGGGVLLPDVETPSEEDCMFGNGKGYRGKRATTVTGTPCQDWAAQEPHRHSIFTPETNPRAGLEKNYCRNPDGDVGGPWCYTTNPRKLYDYCDVPQCAA, the molecular weight of the human plasminogen K5 is 17kDa and the targeting property andthe specificity of tumor of the protein is obviously improved by five times compared with wild type K5. The preparation method comprises the following steps: (1) designing and constructing a RGD-K5 sequence; and (2) expressing, purifying and identifying the RGD-K5 protein.

Description

technical field [0001] The invention relates to a human plasminogen, in particular to an anti-tumor protein-RGD modified human plasminogen Kringle 5 and its preparation method and application. Background technique [0002] The damage to the tissues and organs of the whole body caused by the abnormal proliferation of new blood vessels is one of the most difficult medical problems at present, and it is the etiology or / and important pathological feature of many major diseases in the whole body. Human plasminogen Kringle 5 (Human Plasminogen Kringle 5, K5) is one of the striking angiogenesis-inhibiting proteins. Specificity, small molecular weight, small side effects, relatively stable properties and many other significant advantages (1, Cao.Y, Chen.A.An, S.S.Ji, R.W, Davidson.D and Llinas.M.Kringle 5 of plasminogen is a novel inhibitor of endothelial cell growth. J Biol Chem, 272(36): 22924-22928, 1997; 2, W.R.Ji, L.G.Barrientos, M.Llinás, H.Gray, X.Villarreal, M.E.DeFord, F.J...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C12N15/81A61K47/48A61K38/48A61P35/00C12R1/84A61K47/64
Inventor 金光辉王洋郭中强葛庆王恩华苏文松谭聪
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com