Method for lightening stress response of juvenile plectorhinchus niger in manual operation
A technology of stress response and manual operation, which is applied in application, fish farming, climate change adaptation, etc. It can solve problems such as stabbing operators, surface injuries of fish, and physiological dysfunction of aquatic animals.
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Embodiment 1
[0017] In August 2009, in the seed cultivation workshop of Yantai Baijia Aquatic Products Co., Ltd. located in Huangshanguan Town, Longkou City, Yantai, the water temperature was 22-23°C, salinity 29-30, pH 8.0-8.2, DO>6mg / L For seawater, a 20L plastic storage box is used as a container, and the volume of seawater is 10L, and a small amount of air is used. The juveniles of black rock perch have a total length of 55.2 mm ± 6 mm (average total weight 3.17 g) and are fasted for one day.
[0018] Use 2-phenoxyethanol produced by BASF Chemical Company in Germany, and calculate according to the concentration of 250 μL / L, then 2.5 mL of 2-phenoxyethanol should be added to 10 L of seawater.
[0019] Use a net to fish the juvenile black rock perch into the 2-phenoxyethanol seawater solution. Within 3 minutes, the juvenile fish lie on the bottom of the tank, and then perform operations such as growth measurement, immunization injection, individual labeling or experiments. Bass juvenile...
Embodiment 2
[0022] In September 2009, in the seedling cultivation workshop of Yantai Baijia Aquatic Products Co., Ltd. located in Huangshanguan Town, Longkou City, Yantai, seawater with a water temperature of 21°C, a salinity of 29-30, a pH of 8.0-8.2, and a DO>6mg / L was used , with a 200LPVC tank as a container, with a seawater volume of 100L, and a small amount of air. The juveniles of black rock perch have a total length of 99.0mm±7mm (average total weight 14.9g), and they are fasted for one day.
[0023] Using 2-phenoxyethanol produced by BASF Chemical Company in Germany, calculated according to the concentration of 350 μL / L, then 35 mL of 2-phenoxyethanol should be added to 100 L of seawater.
[0024] Use a net to fish the juvenile black perch into the seawater solution of 2-phenoxyethanol. Within 3 minutes, the juveniles lie on the bottom of the tank, and then they can perform operations such as growth measurement, immunization injection, individual labeling and experiments. The ju...
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