Arthrobacter HW08 for degrading swainsonine and application thereof
A technology of swainsonine and Arthrobacter, applied in the field of microorganisms, can solve the problems of difficult enzyme gene screening and research, low degradation ability, etc., achieve stable degradation characteristics and solve poisoning problems
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Embodiment 1
[0030] Example 1: Isolation of Arthrobacter HW08
[0031] 1) Embed 500 g of Oxytropis chinensis collected from Nanhua Mountain in Haiyuan County, Ningxia Hui Autonomous Region, into the soil at a depth of 50 cm for 6 months.
[0032] 2) take out 500g of soil around the grass sample, and fully mix; get 10g of fully mixed soil samples, dissolve in 50ml of PBS (pH7.2), and stir and mix with a magnetic stirrer, and the supernatant is used as an enriched inoculum (Harder, 1981; Cook et al, 1983).
[0033]3) Adding SW as the only carbon source to the inorganic salt culture solution, so that the final concentration of SW is 10%.
[0034] 4) Take 1ml of the inoculum and add it to 250ml of inorganic salt culture solution containing 10% SW, culture at 30°C and 120rpm for 7 days; take 0.5ml and inoculate it into 50ml of fresh culture solution, and cultivate it for 4 days; repeat this 8 times; finally transfer to Solid inorganic salt culture medium and nutrient plate containing 10% SW. ...
Embodiment 2
[0040] Example 2: Identification of Arthrobacter HW08
[0041] 1. Morphological characteristics
[0042] The colony of the HW08 strain was round, milky white at first, and gradually turned into light yellow with the prolongation of the culture time, with a smooth, moist, raised surface, neat edges, and opaque ( figure 1 ). The shape of the bacteria undergoes a significant change during the growth cycle with the change of medium and culture time. Within 48 hours of culture in inorganic salt medium, they basically belong to rod-shaped cells, with a size of 0.3-0.5 μm×0.5-1.0 μm, and some are irregularly branched rod-shaped or arranged in a unique "V" or "Y" shape. ( figure 2 , image 3 ); After 48 hours of cultivation, it gradually becomes spherical, with a diameter of about 0.6-0.8 μm. Once transferred to fresh medium, the spheroid cells begin to sprout again, becoming again actively growing bacilli with rudimentary branches but no true mycelium, no capsule, no spores, no...
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