Arthrobacter HW08 for degrading swainsonine and application thereof

A technology of swainsonine and Arthrobacter, applied in the field of microorganisms, can solve the problems of difficult enzyme gene screening and research, low degradation ability, etc., achieve stable degradation characteristics and solve poisoning problems

Inactive Publication Date: 2010-06-09
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhao Xinghua et al. (2008) used these research methods to isolate and screen two degrading bacteria (YLZZ-1 and YLZZ-2) th

Method used

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  • Arthrobacter HW08 for degrading swainsonine and application thereof
  • Arthrobacter HW08 for degrading swainsonine and application thereof
  • Arthrobacter HW08 for degrading swainsonine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation of Arthrobacter HW08

[0031] 1) Embed 500 g of Oxytropis chinensis collected from Nanhua Mountain in Haiyuan County, Ningxia Hui Autonomous Region, into the soil at a depth of 50 cm for 6 months.

[0032] 2) take out 500g of soil around the grass sample, and fully mix; get 10g of fully mixed soil samples, dissolve in 50ml of PBS (pH7.2), and stir and mix with a magnetic stirrer, and the supernatant is used as an enriched inoculum (Harder, 1981; Cook et al, 1983).

[0033]3) Adding SW as the only carbon source to the inorganic salt culture solution, so that the final concentration of SW is 10%.

[0034] 4) Take 1ml of the inoculum and add it to 250ml of inorganic salt culture solution containing 10% SW, culture at 30°C and 120rpm for 7 days; take 0.5ml and inoculate it into 50ml of fresh culture solution, and cultivate it for 4 days; repeat this 8 times; finally transfer to Solid inorganic salt culture medium and nutrient plate containing 10% SW. ...

Embodiment 2

[0040] Example 2: Identification of Arthrobacter HW08

[0041] 1. Morphological characteristics

[0042] The colony of the HW08 strain was round, milky white at first, and gradually turned into light yellow with the prolongation of the culture time, with a smooth, moist, raised surface, neat edges, and opaque ( figure 1 ). The shape of the bacteria undergoes a significant change during the growth cycle with the change of medium and culture time. Within 48 hours of culture in inorganic salt medium, they basically belong to rod-shaped cells, with a size of 0.3-0.5 μm×0.5-1.0 μm, and some are irregularly branched rod-shaped or arranged in a unique "V" or "Y" shape. ( figure 2 , image 3 ); After 48 hours of cultivation, it gradually becomes spherical, with a diameter of about 0.6-0.8 μm. Once transferred to fresh medium, the spheroid cells begin to sprout again, becoming again actively growing bacilli with rudimentary branches but no true mycelium, no capsule, no spores, no...

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Abstract

The invention discloses Arthrobacter sp. HW08 for degrading swainsonine, which is preserved in a common microbe center of the China Microbial Culture Preservation Commission in September 28th, 2009 and has the preservation number of CGMCC No. 3313. The bacterial colony of a bacterial strain is in a circular shape and gradually changed into faint yellow from oyster white with the prolongation of culture time, has a smooth, moist and raised surface and neat edges and is non-transparent; and the bacterial strain is cultured within 48 hours in an inorganic salt culture medium, basically belongs to a stab cell, has the size of 0.3-0.5 micrometer*0.5-1.0 micrometer, is gradually changed into a ball shape after cultured for 48 hours and has the diameter about 0.60-0.8 micrometer. The bacterial strain is obtained by separating and screening from soil embedding yellowflower crazyweed herbs (loco weeds) and has the capacity of efficiently degrading the swainsonine, i.e. toxins of the loco weeds.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to an Arthrobacter sp., in particular to an Arthrobacter sp. capable of efficiently degrading a locotoxin—Swainsonine (SW) and an application thereof. Background technique [0002] Locoweed is a general term for the poisonous plants of the genus Oxytropis and Astragalus of the leguminous family, and it is the most serious poisonous weed that endangers the sustainable development of grassland animal husbandry production in the world. There are currently 45 species of locoweed poisonous plants in my country (22 species of Astragalus and 23 species of Oxytropis genus), 12 species of which pose serious hazards, including Oxytropis japonica, Oxytropis florida, Oxytropis gansu, Oxytropis pubescens, and Oxytropis japonicus. Oxytropis glacis, Oxytropis glacier, Oxytropis baotou, Oxytropis broad-bract, Huaxi Oxytropis, Variation Astragalus, Astragalus stemstratum, Astragalus multiflora, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61K48/00A61P39/02C12R1/06
Inventor 王建华胡延春王妍
Owner NORTHWEST A & F UNIV
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