Staining fluid and method for rapidly detecting novel cryptococcus

A Cryptococcus neoformans, staining solution technology, applied in microorganism-based methods, biochemical equipment and methods, microbial determination/inspection, etc. Short dyeing time and excellent dyeing effect
CN101724682AInactive Publication Date: 2010-06-09东阳市人民医院

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
东阳市人民医院
Publication Date
2010-06-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to staining fluid and a method for rapidly detecting novel Cryptococcus. The staining fluid comprises 0.5-3.0g of AZO-blue, 1.0-10.0g of glacial acetic acid and 90-110g of distilled water. The method for rapidly detecting novel cryptococcus comprises the steps of preparation of the staining fluid, mixing, detection, counting and the like. The invention has the advantages of simple, convenient, rapid, sensitive and accurate detection, high specificity, easy preservation of the staining fluid, short staining time and excellent staining effect.
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Description

Technical field

[0001] The invention belongs to the detection field of Cryptococcus neoformans, in particular to a staining solution and method for rapidly detecting Cryptococcus neoformans. Background technique

[0002] The diagnosis of Cryptococcus neoformans currently mainly relies on ink smears and fungal culture, and the diagnosis is based on the detection of pathogens. In terms of rapid diagnosis, although direct smear ink staining is simple, it is relatively difficult to observe under the microscope, so the positive detection rate is low. Foreign literature reports that the positive rate is only 53%~56% even in the case of multiple inspections. There are certain false positives and it is difficult to count cryptococcus. Traditional fungal culture usually takes several days. Although the positive rate of culture can reach more than 90%, the diagnosis is delayed due to the long cycle. Animal inoculation can detect some patients with high clinical suspicion and negative sme...

Claims

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