Staining fluid and method for rapidly detecting novel cryptococcus

Abstract

The invention relates to staining fluid and a method for rapidly detecting novel Cryptococcus. The staining fluid comprises 0.5-3.0g of AZO-blue, 1.0-10.0g of glacial acetic acid and 90-110g of distilled water. The method for rapidly detecting novel cryptococcus comprises the steps of preparation of the staining fluid, mixing, detection, counting and the like. The invention has the advantages of simple, convenient, rapid, sensitive and accurate detection, high specificity, easy preservation of the staining fluid, short staining time and excellent staining effect.

Description

Technical field [0001] The invention belongs to the detection field of Cryptococcus neoformans, in particular to a staining solution and method for rapidly detecting Cryptococcus neoformans. Background technique [0002] The diagnosis of Cryptococcus neoformans currently mainly relies on ink smears and fungal culture, and the diagnosis is based on the detection of pathogens. In terms of rapid diagnosis, although direct smear ink staining is simple, it is relatively difficult to observe under the microscope, so the positive detection rate is low. Foreign literature reports that the positive rate is only 53%~56% even in the case of multiple inspections. There are certain false positives and it is difficult to count cryptococcus. Traditional fungal culture usually takes several days. Although the positive rate of culture can reach more than 90%, the diagnosis is delayed due to the long cycle. Animal inoculation can detect some patients with high clinical suspicion and negative sme...

AI Technical Summary

Problems solved by technology

Animal inoculation can detect some patients with high clinical suspicion but negative smears and cultures. This method takes longer and has little significance for rapid diagnosis. It is rarely used clinically.

In recent years, with the rapid development of molecular biology technology and immunology technology, a new way has been opened up for the diagnosis of Cryptococcus neoformans. Both domestic and foreign reports have confirmed that the use of immunology technology and PCR technology has high accuracy in rapid diagnosis. and sensitivity, but due to the different kits used, the results are often different, and there are certain false positive and false negative rates, and because of the high cost of immunological technology and PCR technology to detect Cryptococcus neoformans, the operation is more complicated than staining microscopy, and it is not easy to detect Cryptococcus neoformans. It belongs to the gold standard for the diagnosis of Cryptococcus neoformans, and it is not conducive to carrying out in townships and grassroots hospitals, so it has not been widely used in the clinical diagnosis of laboratory departments

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