Polygonal plastic bag swing cell reactor and method for culturing animal cells

Abstract

The invention relates to a polygonal plastic bag swing cell reactor and a method for culturing animal cells. The cell reactor solves the problem of self function amplification and intensification in the face of the large-scale high-density culture requirement of animal cells. The polygonal plastic bag swing cell reactor comprises a swing bed (60) which is connected with a swinging mechanism, and a polygonal reaction tank (100) is installed in the swinging mechanism. A polygonal plastic culturing bag (90) is installed in the polygonal reaction tank, and a plastic multifunctional plate (91) is installed on the polygonal plastic culturing bag (90). A temperature probe (82), a pH probe (81) and a dissolved oxygen probe (80) are respectively installed on the multifunctional plate and penetrated into the polygonal plastic culturing bag. The multifunctional plate is provided with an air inlet (886), an air outlet (83) and a feed inlet (88), and the bottom of the polygonal reaction tank is provided with a distribution awl (105). The invention provides a high quality environment for animal cell culture.

Description

Technical field: [0001] The invention relates to a biological cell reactor, in particular to a polygonal plastic bag swinging cell reactor for animal cell cultivation and a method for cultivating animal cells. Background technique: [0002] At present, the world's biotechnology pharmaceutical industry is developing rapidly and has made great achievements. The expression and production of various diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells has a very broad market application prospect. Therefore, the biological cell reactor used for large-scale high-density cultivation of animal cells has become the most important equipment in the biotechnology pharmaceutical industry. It largely determines the product cost, production scale and product category of biotechnology pharmaceutical companies. The existing reactor adopts a mechanical stirring method inserted into the tank...

AI Technical Summary

Problems solved by technology

The existing reactor adopts a mechanical stirring method inserted into the tank to promote the flow of the liquid. The structure of the stirring device and its lubricating substances often cause microbial contamination during work. Bacterial contamination leads to cell death

In order to increase the dissolved oxygen level, it is usually achieved by blowing gas under the liquid surface, but whether it is mixed gas or pure oxygen, it has its negative effects. Because the bubbles burst on the liquid surface, their explosive force can damage animal cells. Too much pure oxygen can also cause cell poisoning. In addition, when cultivating in medium and small volumes, the reactor cannot be moved due to the stainless steel tank and pipeline of the traditional stirred tank, and the environmental requirements are high, so high-pressure steam must be used for heating and sterilization. , which requires special conditions such as steam source, which greatly limits the versatility and convenience of the reactor

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