Method and kit for rapid detection of ethanol content in microbial fermentation solution

A technology for microbial fermentation broth and detection method, which is applied in the field of quantitative and rapid detection of ethanol, can solve the problems of large sample volume, poor result accuracy, and difficulty in high-throughput detection of multiple samples, and achieves good specificity, high sensitivity and simple method. Effect

Inactive Publication Date: 2010-09-08
WUXI LINGTE BIOTECH
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Problems solved by technology

The specific gravity method requires distillation during analysis, and the sample distillation process is time-consuming and labor-intensive, requires a large sample volume, and is difficult to detect multiple samples at high throughput
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  • Method and kit for rapid detection of ethanol content in microbial fermentation solution
  • Method and kit for rapid detection of ethanol content in microbial fermentation solution
  • Method and kit for rapid detection of ethanol content in microbial fermentation solution

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Embodiment Construction

[0024] The following examples specifically illustrate the detection steps of this patent:

[0025] 1. Prepare DNS and potassium dichromate solution: DNS solution: add 6.3g of 3,5-dinitrosalicylic acid and 262mL of 2mol NaOH solution to 500mL of hot water solution containing 185g of potassium sodium tartrate, then add 5g Crystallized phenol and 5g sodium sulfite, stir to dissolve, after cooling, add distilled water to make the volume to 1000mL, store it in a brown bottle for 1 week and then be stable for use. Potassium dichromate solution: Saturate potassium dichromate with 8N sulfuric acid.

[0026] 2. Prepare ethanol and glucose standard sample solution: Ethanol standard sample solution: add 4mL of absolute ethanol into 96mL of purified water to make a 4% solution, and then use purified water to dilute it twice to 2%, 1%, 0.5% , 0.25%, 0.125%, 0.0625%, 0.03125%, 0.015625%, 0.007813% ethanol solution. Glucose standard sample solution: Weigh 100 mg of analytically pure glucose dri...

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Abstract

The invention discloses a method for rapid detection of ethanol content and reducing sugar content, which belongs to the technical field of quantitative detection of chemical components. The method comprises the following steps of: pre-processing microbial fermentation solution; establishing a reducing sugar standard curve by using DNS and detecting the reducing sugar content in a sample; establishing the reducing sugar standard curve by using potassium dichromate and detecting the sample; and establishing an ethanol standard curve by using the potassium dichromate and calculating the ethanol content in the sample. The method can directly detect the ethanol content in the microbial fermentation solution without using a precise detector, and has the advantages of high precision, fast, simple and convenient operation, less required sample and reagent quantity, low cost, less required instrument and equipment, low requirement on environment, no pollution generated in the detection process, large quantity of samples detected at one time and the like.

Description

Technical field: [0001] The invention belongs to a rapid detection method for the content of ethanol and reducing sugar in the technical scope of quantitative detection of chemical components, and particularly relates to a combination of 3,5-dinitrosalicylic acid (DNS) and reducing sugar A new method for quantitative and rapid detection of ethanol was established by the color reaction of potassium dichromate and the oxidation reaction of reducing sugar and ethanol. Background technique: [0002] Ethanol concentration is an important process parameter in the fermentation of fuel ethanol, wine and other microorganisms, which directly affects the optimization and regulation of the fermentation process and the quality and yield of fermentation products. The rapid and accurate determination of the ethanol content in the fermentation broth is a prerequisite for such research and production. And foundation. [0003] Although there are many ethanol detection methods at present, each metho...

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Application Information

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IPC IPC(8): G01N21/78G01N1/34
Inventor 张鹏孙东旭陈才法丁铁林
Owner WUXI LINGTE BIOTECH
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