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Blood cell apoptosis inducing method

A technology of apoptosis induction and blood cells, which is applied in the direction of blood circulation treatment, etc., to achieve the effect of good stability and simple treatment process

Inactive Publication Date: 2010-09-29
JIANGSU SINOWAYS (ZHONGHUI) MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, my country has not yet established a stable and efficient method for inducing blood cell apoptosis.

Method used

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Examples

Experimental program
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Embodiment Construction

[0008] A constant temperature water tank is fixed on the operating table, and an ultraviolet lamp tube with a UV protective cover is installed above the water tank. A container made of quartz glass is fixed in the water tank through a bracket, and the two sides of the upper part of the container are respectively connected to the ozone gas input pipe and output pipe. .

[0009] Blood cell apoptosis treatment process: Control the temperature of the water in the tank to 42.5±0.1°C, put the container with fresh blood on the stand, and soak the blood below the water level of the tank, and the depth is about 3 / 4 of the container; turn on the ozone The gas generating equipment generates ozone gas, opens the valves of the ozone gas input pipeline and output pipeline, and simultaneously irradiates ultraviolet rays with a wavelength of 253.7nm. Keeping the above conditions stable for 20 minutes can effectively and stably induce blood cell apoptosis.

[0010] Reinfusing the above-mentio...

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Abstract

The invention discloses a blood cell apoptosis inducing method, and relates to the technical field of medical treatment, in particular to the technical field of apoptosis induction of in-vitro blood cells. The technical scheme of the invention is that fresh blood cells are apoptotic under the induction of temperature, ozone and ultraviolet irradiation. Under the irradiation of ultraviolet with the wavelength of 253.7nm, a container which is filled with fresh blood is placed in a water bath at the temperature of 42.5+ / -0.1 DEG C, and the gaseous ozone acts on the blood surface for 20 minutes. After the treatment of the method, the blood cell apoptosis incidence reaches 80 percent, the treatment process is simple and scientific, and the stability is high.

Description

technical field [0001] The invention relates to the field of medical technology, and is the technical field of inducing apoptosis of blood cells in vitro. Background technique [0002] Heart failure is characterized by high morbidity, disability and mortality, and is known as the last unconquered bastion in the field of cardiovascular disease prevention and treatment. Combining various contemporary treatment methods, the 5-year mortality rate may still exceed 50% (men) or 30% (women). In recent years, in the study of the mechanism of heart failure, it has been gradually recognized that inflammatory factors and immune activation are important mechanisms for the gradual deterioration of heart failure. Different scholars have tried a variety of different drugs and biological methods for immune intervention in patients with heart failure, but none of them have achieved significant results. [0003] In the past ten years, the research data of different scholars have shown that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/36
Inventor 张振刚居小平冯海友龚开政
Owner JIANGSU SINOWAYS (ZHONGHUI) MEDICAL TECH CO LTD
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