The invention relates to the technical field of disinfection methods of feeds, and particularly relates to a sterilizing process of an animal feed. The sterilizing process comprises the steps of: a, liquefying high-concentration gaseous ozone by water mist spraying to obtain high-concentration liquid ozone; b, loading a dried feed in a high-pressure sealed disinfection tank, adding the liquid ozone in the high-pressure sealed disinfection tank, uniformly stirring, simultaneously introducing the high-concentration gaseous ozone, and sterilizing by an ultraviolet lamp; c, adding quaternary ammonium salt in the disinfection tank; d, after disinfecting, introducing high-temperature steam in the disinfection tank, and stirring the feed to thoroughly remove ozone residues; and e, adding active bacteria into the disinfection tank for fermenting the animal feed. The sterilizing process is scientific, reasonable and safe, has an environment protection effect, and is strong in maneuverability, is thorough in sterilization, convenient to operate, free of toxicity, odor and irritation and strong in bacterial property as adopting a physical and chemical combined mode for sterilization; nutrient substances in the feed are protected from being damaged, nutritional ingredients of the feed increase, the feed is safe and reliable, and no economic loss is caused.