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Flow electroporation device and system

A flow electroporation and electroporation technology, applied in biochemical equipment and methods, biochemical instruments, stress-stimulated microbial growth methods, etc., can solve problems such as reducing efficiency, not completely continuous work, and not being able to achieve high-throughput operations , to achieve the effect of reducing equipment cost

Active Publication Date: 2010-10-13
ETTA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This increases the difficulty of designing the power supply system and is not conducive to energy saving and environmental protection
At the same time, this system does not work completely continuously, and the electroporation chamber needs to be filled-electroporated-evacuated-refilled-electroporated
This way of working reduces efficiency and cannot achieve true high-throughput operations

Method used

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  • Flow electroporation device and system
  • Flow electroporation device and system
  • Flow electroporation device and system

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Embodiment Construction

[0053] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0054] 1 Substrate:

[0055] Because the substrate acts as a support and acts as insulation and isolation between electrodes, the substrate needs to be made of insulating material, or supported by a non-insulating material covered with an insulating layer. The substrate according to the present invention can be made from any solid substrate suitable for the above conditions, preferably those substrates which can be molded or machine cut to the required specifications. Transparent glass or polymer is particularly preferred, since in this case the state of the cells can be observed in real time through a microscope.

[0056] exist figure 2 In the preferred embodiment shown, the substrate is made of ordinary glass, with a length of 4 cm, a width of 2.5 cm, and a height of 2 mm. The size of the substrate can be determined by specific requirement...

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Abstract

The invention discloses a flow electroporation device and a system. The system comprises a flow electroporation device, an injection pump and a voltage source, wherein the flow electroporation device comprises a substrate, electrodes arranged on the substrate, a channel for limiting fluid to flow, a top cover of an inlet and an outlet of fluid, the electrodes are arranged in a crossing way; each pair of electrodes comprise an anode and an cathode which are oppositely arranged, and the channel is arranged on the electrodes; the top cover is arranged above the channel, and the injection pump isconnected to the inlet and the outlet of the top cover in the flow electroporation device through a pipeline and is used for controlling the flow rate of the fluid; and the voltage source is connected with the electrodes through electric connecting pieces and is used for setting and producing pulse voltage. The flow electroporation system utilizes the fluid channel and the injection pump connected with the fluid channel to realize the continuous flow of various suspensions in the fluid channel, so that the fast treatment of a large number of samples can be continuously realized in the processthat cells are electroporated.

Description

technical field [0001] The present invention relates to a flow-type electroporation device, and more specifically, the present invention relates to a system for efficiently utilizing the flow-type electroporation device for electroporation. Background technique [0002] The cell membrane is a layer of thin film surrounding the periphery of the cell, which is a permeability barrier for the selective material exchange between the cell and the outside world. The cell membrane makes the cell an independent living unit and has a relatively stable internal environment. Some substances in the surrounding environment can pass through the cell membrane, while others cannot. Cells can absorb nutrients from the surrounding environment through the cell membrane, discharge metabolites, and make the transport of substances reach a state of balance. Therefore, the basic function of the cell membrane is to maintain the relative stability of the intracellular microenvironment and selective...

Claims

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Application Information

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IPC IPC(8): C12M1/42
CPCC12M23/38C12M35/02
Inventor 李志宏梁子才王玮魏泽文黄璜
Owner ETTA BIOTECH
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