Biological flora suspending agent for degrading petroleum and preparation method and application method thereof

A technology of biological bacteria and suspending agent, applied in the direction of biochemical equipment and methods, methods based on microorganisms, chemical instruments and methods, etc., can solve the problems of difficulty in ensuring the stability of the bacteria group and the low degradation rate of the bacteria group, and achieve simple structure , easy to control, avoiding the effect of processing capacity limitations

Active Publication Date: 2013-02-27
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation rate of the flora involved in this prior art is low, and it is difficult to ensure the stability of the flora in the process of amplification and subculture of the natural flora

Method used

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  • Biological flora suspending agent for degrading petroleum and preparation method and application method thereof
  • Biological flora suspending agent for degrading petroleum and preparation method and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Treatment of Petroleum Wastewater by Artificially Constructed Bacteria

[0046] Select Bacillus sp. DQ5CCTCC M 2010078, Bacillus sp. DQ10CCTCC M 2010079, Enterobacter sp. DQ11CCTCC M 2010084, Bacillus sp. DQ16CCTCC M 2010080, Bacillus sp. .) DQ26CCTCC M2010081, Bacillus sp. DQ62CCTCC M 2010082 and Bacillus sp. DQ85CCTCC M 2010083 strains were operated as follows:

[0047] (1) Slant culture: inoculate the above-mentioned strains on LB solid slant medium, and culture the bacteria at 30±1°C for 18 to 24 hours;

[0048] (2) First-level seed cultivation: put the bacteria cultured in step (1) into 100mL LB liquid medium with an inoculation loop under sterile conditions, and inoculate at 120-200 rpm under the condition of 30±1°C Vibrating culture on a shaker for 12-18 hours to the middle of the logarithmic growth phase to obtain first-class seeds;

[0049] (3) Expansion cultivation: with the inoculum amount of 5% to 8% by volume, connect the first-grade seeds in 1000mL LB li...

Embodiment 2

[0060] Treatment of Petroleum Wastewater by Artificially Constructed Bacteria

[0061] Select Bacillus sp. DQ5CCTCC M 2010078, Bacillus sp. DQ10CCTCC M 2010079, Enterobacter sp. DQ11CCTCC M 2010084, Bacillus sp. DQ16CCTCC M 2010080, Bacillus sp. .) DQ26CCTCC M2010081, Bacillus sp. DQ62CCTCC M 2010082 and Bacillus sp. DQ85CCTCC M 2010083 strains were operated as follows:

[0062] (1) Slant culture: inoculate the above-mentioned strains on LB solid slant medium, and culture the bacteria at 30±1°C for 18 to 24 hours;

[0063](2) First-level seed cultivation: put the bacteria cultured in step (1) into 100mL LB liquid medium with an inoculation loop under sterile conditions, and inoculate at 120-200 rpm under the condition of 30±1°C Vibrating culture on a shaker for 12-18 hours to the middle of the logarithmic growth phase to obtain first-class seeds;

[0064] (3) Expansion cultivation: with the inoculum amount of 5% to 8% by volume, connect the first-grade seeds in 1000mL LB liq...

Embodiment 3

[0075] Treatment of Petroleum Wastewater by a Single Strain

[0076] Select Bacillus sp. DQ5CCTCC M 2010078, Bacillus sp. DQ10CCTCC M 2010079, Enterobacter sp. DQ11CCTCC M 2010084, Bacillus sp. DQ16CCTCC M 2010080, Bacillus sp. .) DQ26CCTCC M2010081, Bacillus sp. DQ62CCTCC M 2010082 and Bacillus sp. DQ85CCTCC M 2010083 strains were operated as follows:

[0077] (1) Slant culture: inoculate the above-mentioned strains on LB solid slant medium, and culture the bacteria at 30±1°C for 18 to 24 hours;

[0078] (2) First-level seed cultivation: put the bacteria cultured in step (1) into 100mL LB liquid medium with an inoculation loop under sterile conditions, and inoculate at 120-200 rpm under the condition of 30±1°C Vibrating culture on a shaker for 12-18 hours to the middle of the logarithmic growth phase to obtain first-class seeds;

[0079] (3) Expansion cultivation: with the inoculum amount of 5% to 8% by volume, connect the first-grade seeds in 1000mL LB liquid medium, and s...

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Abstract

The invention relates to a biological flora suspending agent for degrading petroleum and a preparation method and an application method thereof. The biological flora suspending agent consists of the following components in part by volume: 1 to 2 parts of DQ5 bacillus suspension, 1 part of DQ10 bacillus suspension, 1 to 2 parts of DQ11 enterobacter suspension, 1 part of DQ16 bacillus suspension, 0.5 to 1 part of DQ26 bacillus suspension, 1 part of DQ62 bacillus suspension and 0.5 to 1 part of DQ85 bacillus suspension. Compared with a single bacterial strain, the biological flora suspending agent has the advantages of simple building method, easy control, high repeatability and easy culture of selected bacterial strains.

Description

technical field [0001] The invention relates to a suspending agent in the technical field of biodegradation treatment and a preparation and application method thereof, in particular to a biological flora suspending agent for degrading petroleum and a preparation and application method thereof. Background technique [0002] Petroleum is an important energy and chemical resource. With the development of modern industry, the demand for oil is increasing day by day. Now the world's annual oil output is more than 3 billion tons, and in the process of mining, transportation, refining and use, due to various reasons, oil spills and their wastes have caused serious pollution to soil and water resources. Especially in recent decades, the discovery and exploitation of submarine oil fields and the frequent occurrence of oil tanker accidents have caused serious pollution to the ocean. The soil around the production wells of Daqing Oilfield and Huabei Oilfield is seriously polluted by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/01C12R1/07C02F103/10
Inventor 许平马翠卿张正芝杨春玉唐鸿志
Owner SHANGHAI JIAO TONG UNIV
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