Promoter of efficient expression Zmzf gene for water flooding of corn root
A promoter and root technology, applied in the field of plant genetic engineering
Inactive Publication Date: 2012-01-04
YANGTZE UNIVERSITY
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Problems solved by technology
The cloning of the Zmzf gene promoter has not been reported yet
Method used
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Embodiment Construction
[0071] The promoter of the Zmzf gene was cloned into the transformation vector, and then a Tnos terminator was connected to construct a transformation vector. The transformed target gene is cloned between the Zmzf gene promoter and the Tnos terminator in the correct direction to construct a transformation vector. Then, the target gene is transformed into recipient cells by gene gun bombardment method or Agrobacterium-mediated method, and transgenic plants are obtained by technical means such as tissue culture. Under waterlogging and flooding conditions, the Zmzf gene promoter can promote the high-efficiency expression of downstream target genes in plant roots.
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The invention belongs to the technical field of plant gene engineering and is characterized by cloning a promoter of an efficient expression Zmzf gene for water flooding of a corn root from a corn inbred line Mo17; and the nucleotide sequence of the promoter is shown as SEQ ID No. 1. By connecting the promoter of the Zmzf gene with target genes of insect resistance, disease-resistance, resilience and the like and applying the genes to genetic transformation of xerophyte, the promoter can promote the efficient expression of the downstream target gene under the anaerobic conditions of water-logging, water flooding and the like and improve the capacities of the root of a transgenic plant, such as insect resistance, disease-resistance, resilience and the like; and meanwhile, the promoter is only expressed at the root and does not relate to the problem of food safety of the part of an edible transgenic plant above the ground; and thus the promoter has good application prospect in genetic improvement on water-logging tolerance of the xerophyte.
Description
Technical field: [0001] The invention relates to a promoter for highly expressing Zmzf gene in waterlogged corn roots, which belongs to the technical field of plant genetic engineering. Background technique: [0002] Spring maize in southern my country and summer maize in northern China often suffer from floods and waterlogging during the normal cultivation season, and the maize root system is in a low oxygen state for a long time, resulting in a 10%-40% reduction in maize yield. There are generally two ways to create new germplasms of waterlogging-tolerant maize. One is to discover and utilize favorable genes in existing maize germplasms or their close relatives, through breeding methods such as hybridization and backcrossing, or biotechnology such as molecular marker-assisted selection. The method is to introduce genes related to waterlogging resistance into the existing excellent maize breeding materials. However, due to the lack of waterlogging-tolerant resources of ma...
Claims
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IPC IPC(8): C12N15/11
Inventor 杜何为张祖新黄敏许先凤
Owner YANGTZE UNIVERSITY
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