Production method of roegneria kamoji seeds
A production method, the technology of goose watching grass, applied in the fields of seed and rhizome treatment, application, agriculture, etc., to achieve the effect of small physiological damage, wide mutation spectrum and high mutation frequency
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Embodiment 1
[0016] Goose view grass 05-11 material, seed water content 10%, put the seeds on the plate of the ion beam implantation device, the seed embryos are stuck on the plate with double-sided adhesive in the way of spiral outward with the center of the plate as the center of the circle; after that, Put the plate into the target chamber for low-energy nitrogen ion beam implantation, the implantation dose of low-energy nitrogen ion beam is 4000×2.6×10 13 ions / cm 2 , the energy in the target chamber is 25 keV, the nitrogen ion beam current is 12 mA, intermittent pulse injection is adopted, the dose of each pulse is 100, the interval between each injection is 30s, and the vacuum degree during injection is 2.6× 10 -2 Pa; Greenhouse seedling cultivation within one week after injection, the germination index of 05-11 was 21.43% higher than that of untreated seeds, and the seedlings were transplanted in the Shaerqin Grass Resources Key Field Scientific Observation and Experiment Station of...
Embodiment 2
[0018] Goose view grass 05-28 material, seed water content 6%, put the seeds on the plate of the ion beam implantation device, the seed embryos are stuck on the plate with double-sided adhesive in the way of spiral outward with the center of the plate as the center of the circle; after that, Put the plate into the target chamber for low-energy nitrogen ion beam implantation, the implantation dose of low-energy nitrogen ion beam is 3500×2.6×10 13 ions / cm 2 , the energy in the target chamber is 25 keV, the nitrogen ion beam current is 12 mA, intermittent pulse injection is adopted, the dose of each pulse is 100, the interval between each injection is 30s, and the vacuum degree during injection is 2× 10 -2 Pa; Greenhouse seedling cultivation within one week after injection, the germination index of 05-28 was 10.93% lower than that of untreated seeds, and after 50 days of germination, the height of seedlings was 9.17% higher than that of untreated seeds, and the seedlings were tr...
Embodiment 3
[0020] Goose View Grass 05-28 material, seed water content 13%, place the seeds on the plate of the ion beam implantation device, the seed embryos are stuck on the plate with double-sided adhesive in the form of spiral outward rotation with the center of the plate as the center; after that, Put the plate into the target chamber for low-energy nitrogen ion beam implantation, the implantation dose of low-energy nitrogen ion beam is 700×2.6×10 13 ions / cm 2 , the energy in the target chamber is 25 keV, the nitrogen ion beam current is 12 mA, intermittent pulse injection is adopted, the dose of each pulse is 100, the interval between each injection is 30s, and the vacuum degree during injection is 1.6× 10 -2 Pa; Greenhouse seedling cultivation within one week after injection, the germination index of 05-28 was 2.26% higher than that of untreated seeds, and after 50 days of germination, the height of seedlings was 5.21% higher than that of untreated seeds, and the seedlings were tr...
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