Kit for diagnosing post-kidney transplant acute rejection reaction

Abstract

The invention relates to a kit for diagnosing post-kidney transplant acute rejection reaction, which is characterized by consisting of bioproteins, enzyme-labelled antibody of the bioprotein, carbonate buffer solution with a pH value of 9.6, phosphate buffer solution with a pH value of 7.4, haemocyanin diluents, stop solution, tetramethylbenzidine substrate solution, normal human serum and positive control serum, wherein the bioproteins are 20 bioproteins including protein S100-A8, protein S100-A9, ITIH3 precursor, IGFBP2 precursor, hemopexin precursor and the like. The invention provides the use of the 20 bioproteins including complements, cell factors, fibrin, binding proteins and other various bioproteins or the use of the combination of the 20 bioproteins with other clinical indexes as the main index for diagnosing the post-kidney transplant acute rejection reaction.

Description

technical field [0001] The invention relates to a kit for diagnosing acute rejection after kidney transplantation, belonging to the field of biotechnology. Background technique [0002] Rejection after kidney transplantation is a series of humoral and cellular immune reactions that occur after the recipient's immune system recognizes allograft kidney antigens. The distinction between hyperacute, acute and chronic rejection is mainly based on the time of occurrence and the speed of disease progression. . Acute rejection can occur at any time after transplantation, and is most common within the first month. The pathogenesis of acute rejection is mostly delayed allergic cellular immunity, and its pathology mainly has the following two types: the main cellular rejection is mainly renal interstitial edema and a large number of immune active cell infiltration, and the humoral rejection is mainly Most of the main cases are fibrinoid necrosis and vasculitis of the vessel wall of m...

AI Technical Summary

Problems solved by technology

Secondly, renal biopsy is mostly the choice after excluding the possibility of other diseases, and the examination and diagnosis in the process of excluding will take a lot of time, resulting in unnecessary economic losses and waste of medical resources

Furthermore, the procedures of renal biopsy are relatively cumbersome, and there are related requirements for pre-examination, surgical operation, and post-processing, which makes it inconvenient to perform. Therefore, regular follow-up renal biopsy is often difficult for kidney transplant patients.