Kit for diagnosing post-kidney transplant acute rejection reaction

An acute rejection and kit technology, applied in the biological field, can solve the problems of cumbersome renal biopsy procedures, time-consuming examination and diagnosis, and unacceptable problems.

Inactive Publication Date: 2011-01-05
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

Secondly, renal biopsy is mostly the choice after excluding the possibility of other diseases, and the examination and diagnosis in the process of excluding will take a lot of time, resulting in unnecessary economic losses and waste of medical resources
Furthermore, the pro...

Method used

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Embodiment

[0012] A kit for diagnosing acute rejection after kidney transplantation, consisting of biological protein and its enzyme-labeled antibody, carbonate buffer solution with a pH value of 9.6, phosphate buffer solution with a pH value of 7.4, serum protein diluent, Stop solution, tetramethylbenzidine substrate solution, normal human serum and positive control serum;

[0013] Wherein, the biological protein includes protein S100-A8, protein S100-A9, ITIH3 precursor, IGFBP2 precursor, hemopexin precursor, A1BG precursor, APOA4 precursor, fibrinogen β chain precursor, Haptoglobin precursor, COL1A2 precursor, ITIH1 precursor, COL1A1 precursor, SERPINC1 antithrombin III variant, SERPINC1 protein, fibronectin 1 isoform preproprotein, SERPINA3, C3 precursor, C9 pre 20 kinds of biological proteins including fibrinogen alpha chain isoform precursor and vitamin D binding protein precursor.

[0014] The carbonate buffer solution with a pH value of 9.6 was obtained by adding 1.59 g of sodiu...

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Abstract

The invention relates to a kit for diagnosing post-kidney transplant acute rejection reaction, which is characterized by consisting of bioproteins, enzyme-labelled antibody of the bioprotein, carbonate buffer solution with a pH value of 9.6, phosphate buffer solution with a pH value of 7.4, haemocyanin diluents, stop solution, tetramethylbenzidine substrate solution, normal human serum and positive control serum, wherein the bioproteins are 20 bioproteins including protein S100-A8, protein S100-A9, ITIH3 precursor, IGFBP2 precursor, hemopexin precursor and the like. The invention provides the use of the 20 bioproteins including complements, cell factors, fibrin, binding proteins and other various bioproteins or the use of the combination of the 20 bioproteins with other clinical indexes as the main index for diagnosing the post-kidney transplant acute rejection reaction.

Description

technical field [0001] The invention relates to a kit for diagnosing acute rejection after kidney transplantation, belonging to the field of biotechnology. Background technique [0002] Rejection after kidney transplantation is a series of humoral and cellular immune reactions that occur after the recipient's immune system recognizes allograft kidney antigens. The distinction between hyperacute, acute and chronic rejection is mainly based on the time of occurrence and the speed of disease progression. . Acute rejection can occur at any time after transplantation, and is most common within the first month. The pathogenesis of acute rejection is mostly delayed allergic cellular immunity, and its pathology mainly has the following two types: the main cellular rejection is mainly renal interstitial edema and a large number of immune active cell infiltration, and the humoral rejection is mainly Most of the main cases are fibrinoid necrosis and vasculitis of the vessel wall of m...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/558
Inventor 朱同玉王向东武多娇朱冬戎瑞明许明
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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