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HLA-DR9 restrictive regulatory T cell epitope of Hepatitis B virus core antigen and e antigen and application thereof

A technology of HLA-DR9 and core antigens, applied in the field of regulatory T cell epitopes, to achieve the effect of rebuilding cellular immune function, breaking HBV immune tolerance, and efficiently inhibiting and clearing

Inactive Publication Date: 2013-01-30
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether protein antigens derived from HBV such as HBcAg and HBeAg are involved in the induction and maintenance of CHB-related Tregs and their related Treg epitopes has not been reported yet.

Method used

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  • HLA-DR9 restrictive regulatory T cell epitope of Hepatitis B virus core antigen and e antigen and application thereof
  • HLA-DR9 restrictive regulatory T cell epitope of Hepatitis B virus core antigen and e antigen and application thereof
  • HLA-DR9 restrictive regulatory T cell epitope of Hepatitis B virus core antigen and e antigen and application thereof

Examples

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Embodiment Construction

[0022] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The concentrations of candidate epitope peptides or irrelevant control peptides, IL-2, anti-CD28, anti-HLA-DR and anti-CD3 antibody (anti-CD3) mentioned in the examples all refer to their final concentrations in the mixture.

[0023] 1. Pan-restricted CD4 + Analysis of T cell epitope hotspots and synthesis of candidate epitope peptides

[0024] Obtain existing CD4 from the epitope database (http: / / www.immunepitope.org / ) +T cell epitopes, according to relevant literature reports and online software MHCPred (http: / / research.i2r.a-star.edu.sg / multipre / ) and SYFPEITHI (http: / / www.syfpeithi.de / ) to predict HBcAg Treg epitopes. Since HBeAg and HBcAg have a common sequence of 70%, HBeAg in serum can be regarded as the secreted type of HBcAg. Therefore, the present invention uses three peptides in the common sequence of HBeAg and HBcAg as candi...

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Abstract

The invention discloses an HLA-DR9 restrictive regulatory T cell epitope of Hepatitis B virus core antigen and e antigen, which is composed of the following amino acid sequence: SRDLVVNYVNTNMGLKIRQLLWFHI. The invention also discloses an application of a regulatory T cell epitope in preparing hepatitis B therapeutic vaccine containing the Hepatitis B virus core antigen and e antigen, namely when the hepatitis B therapeutic vaccine containing the Hepatitis B virus core antigen and / or e antigen is prepared, the regulatory T cell epitope with immunosuppressive action in the Hepatitis B virus core antigen and / or e antigen is eliminated. The invention provides a new strategy and a method for development of the high-efficient hepatitis B therapeutic vaccine, is expected to break hepatitis B immune tolerance, rebuilds cellular immune function and effectively inhibits and eliminates the hepatitis B virus.

Description

technical field [0001] The present invention relates to a regulatory T cell (Treg) epitope, in particular to the HLA-DR9 restricted Treg epitope of hepatitis B virus core antigen (HBcAg) and hepatitis B virus e antigen (HBeAg), and also relates to the application of the Treg epitope . Background technique [0002] Hepatitis B virus (HBV) infection is widespread worldwide and poses a serious threat to human health. Anti-HBV specific immune response plays a decisive role in eliminating HBV from the body. Multiple studies report detection of active virus-specific CD4 in recoverers of acute HBV infection + and CD8 + T cell responses, whereas only weak virus-specific T cell responses were observed in patients with chronic HBV infection (CHB). CD4 + CD25 + Foxp3 + Treg has been shown to suppress other CD4 + T cells and CD8 + The activation and proliferation of T cells play an important role in maintaining normal peripheral immune tolerance. Current studies have shown tha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/02A61K39/29A61P1/16A61P31/20
Inventor 王莉张梦军吴玉章
Owner ARMY MEDICAL UNIV
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