Heat resistance associated molecular marker on chicken heat stress protein 70 gene and acquiring method and application thereof

Abstract

The invention discloses a heat resistance associated molecular marker on chicken heat stress protein 70 gene and an acquiring method and application thereof. The heat resistance associated molecular marker of the invention is obtained from coding regions A+258G and C+1437T of chicken heat stress protein 70 gene. PCR amplification is carried out on heat stress protein 70, amplification product is directly sequenced, so as to screen mononucleotide polymorphic site, correlation analysis is carried out on the obtained parting result and chicken heat resistance shape index, the polymorphic site with obvious correlation is determined to be a heat resistance associated molecular marker, and A+258G and C+1437T are obtained. The invention acquires the heat resistance associated molecular marker, heat resistant chicken can be cultivated, and inheritance breeding method is adopted to thoroughly enhance the tolerance of chicken to high temperature.

Description

technical field The invention relates to the technical field of genetic engineering, in particular to a heat resistance-related molecular marker on the chicken heat stress protein 70 gene, an acquisition method and application thereof. Background technique Molecular marker-assisted selective breeding is an important application of genetic engineering in modern poultry breeding. On-site breeding through molecular markers can not only shorten the breeding time limit, greatly reduce the human and material resource consumption of breeding, but also increase the diversity of molecular markers. The application potential of molecular marker-assisted selection breeding in on-site breeding has been greatly improved. High temperature seasons are likely to cause heat stress in chickens, resulting in slow growth and development, decreased immunity, increased morbidity and mortality, and brought huge economic losses to intensive chicken production. At present, comprehensive measures suc...

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Problems solved by technology

At present, there are relatively few studies on molecular markers for heat adaptability of chickens. Chinese patent application 20091021

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