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Method for establishing experimental model for subclinical hepatic encephalopathy (SHE)

A technology of hepatic encephalopathy and experimental models, applied in the field of biomedicine, to achieve the effect of great clinical significance and value

Inactive Publication Date: 2011-02-02
SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE PUTUO DISTRICT CENT HOSPITAL
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Problems solved by technology

The experimental rat liver cirrhosis model disclosed in the prior art is caused by carbon tetrachloride (CCL4) high fat and low protein compound factors. Whether subclinical liver cirrhosis will occur in this model during the period of pseudolobular formation in the 5th to 6th week? Encephalopathy, so far, there is no relevant report

Method used

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  • Method for establishing experimental model for subclinical hepatic encephalopathy (SHE)
  • Method for establishing experimental model for subclinical hepatic encephalopathy (SHE)

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Experimental program
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Embodiment 1

[0025] 1) Establish a classic animal model of experimental rat liver cirrhosis caused by carbon tetrachloride (CCL4) high fat and low protein compound factors:

[0026] Reagents: carbon tetrachloride (CCL4, produced by Changhai Changjiang Chemical Plant, batch number: 201126, specification: analytically pure); olive oil (Olive oil, produced by Shanghai Double Happiness Spice Auxiliary Factory, batch number: 201022, specification: chemically pure); cholesterol (Cholesterol, produced by Shanghai Boao Biotechnology Co., Ltd., batch number: 201220, specification: analytically pure).

[0027] Preparation of reagents and feed:

[0028] CCL 4 Solution: CCL 4 Dissolved in olive oil to prepare 40% CCL 4 olive oil solution;

[0029] High-fat corn meal feed: 79.5% corn meal + 20% lard + 0.5% cholesterol.

[0030] Experimental animals: 60 SD male rats, SPF grade, weighing 220±14 g (purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences).

[0031] Modeling: 9...

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Abstract

The invention belongs to the field of biomedicine and particularly relates to a method for establishing an experimental model for subclinical hepatic encephalopathy (SHE). In the method of the invention, an experimental rat liver cirrhosis model caused by composite factors such as high fat and low protein of carbon tetrachloride is subjected to learning, recall test and evoked potential test of a nervous system and a brain function and the learning, memory and spatial cognition impairment and nerve electrophysiology test abnormity of the model are monitored, so that the method achieves statistical significance compared with the normal comparison group and a relatively ideal SHE animal model is established. An established experiment animal model provides a platform for the research, diagnosis and treatment of SHE and has high clinically practical meaning and value.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a method for establishing an experimental model, in particular to a method for establishing an experimental animal model of subclinical hepatic encephalopathy Background technique [0002] Liver disease is one of the most common diseases that seriously affect human health today. Subclinical hepatic encephalopathy (SHE) refers to patients with chronic liver disease without obvious clinical manifestations of hepatic encephalopathy. Routine neuropsychiatric function tests are normal, but fine intelligence tests are used. and / or electrophysiological testing can only confirm the diagnosis of hepatic encephalopathy. Hepatic encephalopathy is a common complication of chronic liver disease, and its incidence is very high in patients with liver cirrhosis. It is hidden and difficult to be discovered. Clinical patients usually appear to be normal, but their operating ability and stress ability are ...

Claims

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Application Information

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IPC IPC(8): A61B5/04A61P25/00A61P1/16A61K33/00A61K35/12A61K45/00
Inventor 刘龙民朱喜英梁贤栋陈建杰王灵台高月球虞胜殷佩浩
Owner SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE PUTUO DISTRICT CENT HOSPITAL
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