In-situ hybridization detection kit of DAXX gene as well as detection method and application thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problem that the specific mechanism of apoptosis is not completely clear, and achieve the effects of high sensitivity, strong specificity and convenient operation.

Inactive Publication Date: 2011-03-23
NATUREGEN BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Daxx may play different roles in different apoptotic pathways, but its specific mechanism of action in apoptosis is not fully understood

Method used

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  • In-situ hybridization detection kit of DAXX gene as well as detection method and application thereof
  • In-situ hybridization detection kit of DAXX gene as well as detection method and application thereof

Examples

Experimental program
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Embodiment 1

[0044] An in situ hybridization detection kit for DAXX gene, comprising hybridization probes, markers and synergists, wherein the sequence of the hybridization probes is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0045] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0046] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0047] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0048] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0049] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0050] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0051] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0052] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0053] Chromo...

Embodiment 2

[0095] A kind of DAXX gene in situ hybridization detection method and its kit application

[0096] 1. Specimen processing

[0097] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0098] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0099] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0100] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with conditi...

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Abstract

The invention relates to an in-situ hybridization detection kit of a DAXX (death associated protein) gene, which comprises a hybridization probe and a marker, wherein the sequence of the hybridization probe is as shown in SEQ ID NO.1. The invention also discloses an in-situ hybridization detection of the DAXX gene. In addition, the invention also provides an application of the kit in preparing drugs for detecting acute lymphoblastic leukemia. The invention has the advantage that the kit has the characteristics of high sensitivity and strong specificity. The detection method in the invention is convenient and simple in operation, and can be widely applied and popularized in district-level or city-level hospitals.

Description

【Technical field】 [0001] The present invention relates to a kit, in particular to a DAXX gene in situ hybridization detection kit and its detection method and application 【Background technique】 [0002] Acute lymphoblastic leukemia (ALL) is a neoplastic disease originating from B-lineage or T-lineage lymphoid progenitor cells. Abnormal proliferation and aggregation of primitive cells in the bone marrow inhibits normal hematopoiesis, leading to anemia, thrombocytopenia, and neutropenia. Neutropenia; primitive cells can also invade extramedullary tissues, such as meninges, gonads, thymus, liver, spleen, or lymph nodes, causing corresponding lesions. ALL most commonly occurs in children but can occur at any age. [0003] DAXX is a death cooperating protein, a new type of transcriptional regulatory protein, which belongs to DNA binding protein. Daxx is closely related to physiological or pathological processes such as cell transcription regulation, growth and development, apop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 裘建英张云福
Owner NATUREGEN BIOTECH SHANGHAI
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