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Antrodia camphorata cyclonene compound for restraining growth of bone cancer cells

A technology of tumor cells and compounds, applied in the field of Antrodia antrodia cyclohexenone compounds used to inhibit the growth of bone cancer tumor cells, can solve unpublished specific problems

Inactive Publication Date: 2011-04-06
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although it can be known from many current experiments that the extract of Antrodia Cinnamomea has the aforementioned effects, and its constituents have been analyzed one after another, no specific research has been published on which active ingredients in the extract can contribute to the anticancer effect of Antrodia Cinnamomea. The relevant active ingredients need to be clarified by further experimental research. Therefore, if the ingredients contained in the extract can be found to effectively inhibit tumor growth, it will be beneficial to the research on the mechanism of Antrodia Cintrodia Cintrodia anticancer, and to apply Antrodia Cinnamomea to cancer such as bone Cancer treatment and prevention are of great help

Method used

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  • Antrodia camphorata cyclonene compound for restraining growth of bone cancer cells
  • Antrodia camphorata cyclonene compound for restraining growth of bone cancer cells
  • Antrodia camphorata cyclonene compound for restraining growth of bone cancer cells

Examples

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Effect test

Embodiment 1

[0022] 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separate

[0023] Put about 100 grams of Antrodia antrodia mycelia, fruiting bodies or a mixture of the two into a conical flask, add an appropriate proportion of water and alcohol (70% to 100% alcohol aqueous solution), and store at 20 to 25 ° C. Stir and extract for at least 1 hour, then filter through filter paper and a 0.45 μm filter membrane to collect the extract.

[0024] The Antrodia camphorata extract collected above was analyzed by using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP18 chromatographic column (column), and prepared with methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B). It is mobile phase (mobile phase) (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio is 10% % to 90%; 35 to 40 minutes, the B ratio is 10% to 95%), eluted at a speed ...

Embodiment 2

[0027] Anti-bone cancer tumor cell activity test in vitro

[0028] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , added to human bone cancer tumor cell MG-63 culture medium to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by the known MTT assay, and the bone cancer tumor cell MG-63 is an osteoblast-like fibroblast cell line (osteoblast-like fibroblast cell line), which is derived from human The cancer cells isolated from osteosarcoma are very similar in shape and characteristics to osteoblasts.

[0029] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cy...

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Abstract

The invention relates to a novel application of a compound. In the invention, 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10- cyclododecatriene)-2- cyclonene(4-hydroxy-2,3-dimethoxy-6-mehty-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone) is separated and purified from an antrodia camphorate extract. The cyclonene compound can be applied to restricting the growth of a bone cancer cell and the medical composition for restricting the growth of bone cancer cells.

Description

technical field [0001] The present invention relates to a new application of a compound, in particular to a use of a compound isolated and purified from antrodia cinnamomea (Antrodia cinnamomea) extract to inhibit the growth of bone cancer tumor cells. Background technique [0002] Osteosarcoma, commonly known as bone cancer (Osteosarcoma), is a malignant tumor that grows from the skeletal system, and is the most common type of bone tumor. It will invade adjacent tissues and organs, and may also spread to distant Other organs are more likely to occur in children and adolescents between the ages of ten and twenty, and the incidence rate of males is slightly higher than that of females. The cause of bone cancer is unknown so far. Its primary site and its occurrence at a fast-growing age suggest that it may be related to the increase in the activity of osteoblasts. Genetic factors also seem to play a role in bone cancer. May be associated with chronic inflammation, radiation o...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P35/00A61P19/08
Inventor 刘胜勇温武哲郭茂田
Owner GOLDEN BIOTECH
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