Primers and probes for detecting cow SLC35A3 gene V180F mutation

Abstract

The invention provides primers and specific TaqMan MGB probes for accurately and efficiently detecting SLC35A3 gene V180F mutation bits. When the primers and the probes are used, and the real-time fluorescence quantitative PCR (Polymerase Chain Reaction) technology is utilized, the gene type of the cow SLC35A3 gene V180F mutation bits to be detected can be fast and sensitively detected, so that the cow CVM (Complex Vertebral Malformation) hereditary defect carriers can be accurately sieved. Compared with the traditional detection technology, the primers and the probes have various advantages including high detection speed, high sensitivity and high automation degree, in addition, the types can be judged through one PCR, the whole reaction process is carried out in a sealed tube, and the cross contamination is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to primers and specific TaqMan MGB probes for detecting bovine SLC35A3 gene V180F mutation. Background technique [0002] Genetic defects refer to structural or functional changes in the genetic material in germ cells or fertilized eggs, resulting in abnormal anatomical structures or damage to physiological functions in the developed individual. The frequency of inherited disease genes is higher in dairy cow populations, which is associated with high-intensity selection of dairy cows. After decades of high-intensity breeding of dairy cows in the world, the blood relationship of the breeding bulls is getting closer and closer, which increases the risk of rapid spread of harmful genes. Once the breeding bull has a certain genetic defect, through artificial insemination technology, the genetic defect Genes spread rapidly through the herd. [0003] In recent years, my country has imported...

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the cumbersome steps of existing CVM genetic defect gene detection by introducing restriction site polymerase chain reaction (PIRA-PCR) and polymerase chain reaction-single-strand conformation polymorphism (SSCP) method, Time-consuming, low sensitivity, detection results are easily affected by experimental conditions and other shortcomings, provide a suitable primer and specific TaqMan MGB probe that can accurately and efficiently detect the V180F mutation site of the SLC35A3 gene, using these primers and probes, Using real-time fluorescence quantitative PCR technology, it can quickly and sensitively detect the genotype of the SLC35A3 V180F mutation site of the bovine to be tested, so as to accurately screen the carriers of CVM genetic defects

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