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Marker HCCR-1 protein highly expressed in colon cancer tissue and prognostic marker for colon cancer

A technology for HCCR-1 and colon cancer, applied in the field of biomedicine, can solve the problem of inaccurate prognosis assessment of tumor markers

Inactive Publication Date: 2011-05-11
TIANJIN PEOPLE HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of inaccurate prognosis assessment using tumor markers commonly used in the existing colon cancer prognosis monitoring, and to provide a new marker HCCR-1 protein highly expressed in colon cancer tissue and a colon cancer prognosis marker thing

Method used

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  • Marker HCCR-1 protein highly expressed in colon cancer tissue and prognostic marker for colon cancer
  • Marker HCCR-1 protein highly expressed in colon cancer tissue and prognostic marker for colon cancer
  • Marker HCCR-1 protein highly expressed in colon cancer tissue and prognostic marker for colon cancer

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Embodiment 1

[0015] Example 1, Determination of High Expression of HCCR-1 Protein in Colon Cancer Tissue

[0016] Among them, the HCCR-1 antibody was purchased from Beijing Boaosen Company.

[0017] The present invention uses RT-PCR, Western blot and immunohistochemistry to detect and analyze HCCR-1 at the levels of gene transcription and protein expression. The value of HCCR-1 as a prognostic factor for colon cancer was confirmed by the correlation between the expression of HCCR-1 and the Dukes stage, degree of differentiation and tissue type of colon cancer.

[0018] In order to understand the expression of HCCR-1 in colon cancer tissues, the present invention uses Primer premier 5.0 software to design primers according to the principles of primer design, and the sequences are as follows. HCCR-1 upstream primer: 5'-AATGTACCTGTTTCCCAG-3'; downstream primer: 5'-CAGGACCACGTTGTGCAG-3'. Positive control β-actin upstream primer: 5'-GTGGG GCGCC CCAGG CACCA-3'; downstream primer: 5'-CTTCC ...

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Abstract

The invention discloses a marker HCCR-1 protein highly expressed in colon cancer tissue and a prognostic marker for colon cancer and solves the problem of inaccuracy of prognostic evaluation carried out by adopting a cancer marker which is commonly used in the conventional colon cancer prognostic monitoring. The marker HCCR-1 protein highly expressed in the colon cancer tissue is a protein coded by an amino acid sequence shown by SEQ ID No. 1 in the sequence list. The marker protein can also be a protein with high expression activity in the colon cancer, which is obtained by substituting, losing or adding one or several amino acids in the limited amino acid sequence, and segments thereof. The invention also provides a prognostic marker for the colon cancer, which comprises the marker HCCR-1 protein highly expressed in the colon cancer tissue, and one or more medicaments selected from but not limited to EGFR, VEGF and COX-2 genes and proteins.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a marker protein highly expressed in colon cancer tissue and a colon cancer prognosis marker, wherein the marker includes HCCR-1 protein. Background technique [0002] The morbidity and mortality of colorectal cancer in my country are increasing year by year. The key to improving the long-term survival rate of colorectal cancer is to accurately judge the prognosis and choose the appropriate treatment. The prognosis of colon cancer is closely related to factors such as Dukes stage, degree of differentiation, and tissue type. Tumor markers commonly used in the prognosis monitoring of colon cancer include ras, p53, bcl-2 genes, etc. The mutation or overexpression of these genes is of great significance to the judgment of the prognosis of patients and the selection of treatment options. However, because tumor metastasis involves many complex steps, it is still not accurate to use t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415
Inventor 王弋嘉朱思伟唐涛
Owner TIANJIN PEOPLE HOSPITAL
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