Excrement biomarker for early diagnosis of broiler tibial cartilage dysplasia and application thereof
A biomarker and dysplasia technology, applied in the field of biological detection and diagnosis, to achieve the effect of avoiding stress response, high clinical use and promotion value, and high sensitivity
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[0034] Example 1 Screening of broiler TD early diagnosis fecal biomarkers
[0035] (1) Sample selection: The feces samples of TD broilers (animal room of Henan Agricultural University, Xuchang teaching practice base) were selected, and the feces of healthy broilers (purchased from Kaifeng Xingda Chicken Farm) were used as controls. Use thiram to induce the onset of TD and establish an animal model (Tian Wenxia, Li Jiakui, Bi Dingren, et al. Effect of thiram on tibial chondrodysplasia in broiler chickens [J]. Animal Husbandry and Veterinary Medicine, 2006 (08): 48-49.). Among them, CON is marked as the feces of the control group, and TD is marked as the feces of the TD group.
[0036] (2) Metabolomics sequencing: 50 mg of stool samples were treated with 1000 μL of methanol / water (freezing point, 70%, v / v) and 5 μL of 2-chlorophenylalanine (1 μg / mL). Add two pre-cooled sterile steel balls to the sample mixture at -20°C, homogenize at 30 Hz for 3 minutes after 2 minutes. The ...
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