Excrement biomarker for early diagnosis of broiler tibial cartilage dysplasia and application thereof

A biomarker and dysplasia technology, applied in the field of biological detection and diagnosis, to achieve the effect of avoiding stress response, high clinical use and promotion value, and high sensitivity

Active Publication Date: 2022-02-25
HENAN AGRICULTURAL UNIVERSITY
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most biomarkers are mainly targeted at blood samples, and there are few studies on fecal biomarkers

Method used

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  • Excrement biomarker for early diagnosis of broiler tibial cartilage dysplasia and application thereof
  • Excrement biomarker for early diagnosis of broiler tibial cartilage dysplasia and application thereof
  • Excrement biomarker for early diagnosis of broiler tibial cartilage dysplasia and application thereof

Examples

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Effect test

Embodiment 1

[0034] Example 1 Screening of broiler TD early diagnosis fecal biomarkers

[0035] (1) Sample selection: The feces samples of TD broilers (animal room of Henan Agricultural University, Xuchang teaching practice base) were selected, and the feces of healthy broilers (purchased from Kaifeng Xingda Chicken Farm) were used as controls. Use thiram to induce the onset of TD and establish an animal model (Tian Wenxia, ​​Li Jiakui, Bi Dingren, et al. Effect of thiram on tibial chondrodysplasia in broiler chickens [J]. Animal Husbandry and Veterinary Medicine, 2006 (08): 48-49.). Among them, CON is marked as the feces of the control group, and TD is marked as the feces of the TD group.

[0036] (2) Metabolomics sequencing: 50 mg of stool samples were treated with 1000 μL of methanol / water (freezing point, 70%, v / v) and 5 μL of 2-chlorophenylalanine (1 μg / mL). Add two pre-cooled sterile steel balls to the sample mixture at -20°C, homogenize at 30 Hz for 3 minutes after 2 minutes. The ...

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Abstract

The invention belongs to the technical field of biological detection and diagnosis, and particularly relates to an excrement biomarker for early diagnosis of broiler tibial cartilage dysplasia and application of the excrement biomarker. According to the present invention, differential metabolite 4-hydroxybenzaldehyde capable of distinguishing TD broilers and healthy broilers is obtained through screening based on a metabonomics method and can be used for indicating early TD of the broilers; compared with an existing TD clinical diagnosis method, the method has the advantages that the excrement sample is innovatively selected for clinical detection of a non-invasive biomarker, and the purposes of non-invasion, simplicity, convenience and no stress reaction are achieved; a sample is easy to sample, the detection sample can be collected under a stress-free condition, the stress reaction of the broiler chicken is avoided, and the clinical diagnosis of TD can be simply, conveniently and non-invasively carried out; the excrement biomarker has high sensitivity to early diagnosis of TD, can detect the sub-clinical disease broilers, and is beneficial to intervention and treatment at the early stage of disease.

Description

technical field [0001] The invention belongs to the technical field of biological detection and diagnosis, and in particular relates to a fecal biomarker for the early diagnosis of broiler tibial chondrodysplasia and an application thereof. Background technique [0002] Tibial chondrodysplasia (TD) is a common metabolic cartilage disease that occurs in poultry, especially broiler chickens and turkeys. It is characterized by abnormal development of the bone growth plate, causing deformity of the tibia in broilers, leading to lameness in walking. According to statistics, there are 12.5 billion poultry with leg problems every year in the world. Studies have shown that the impact of TD on the skeletal system of broilers and turkeys is as high as 30% and 90%, respectively. The abnormal growth and development of poultry bones will indirectly lead to a reduction of about 10-40% in the profits of the poultry industry, bringing great economic losses to the poultry industry. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N33/48G16B20/00
CPCG01N30/8696G01N33/483G16B20/00G01N2030/8813
Inventor 黄淑成曹芹芹林露茜张朝栋徐婷婷何彦锋王学兵
Owner HENAN AGRICULTURAL UNIVERSITY
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