45results about How to "No stress response" patented technology

The invention discloses a method for monitoring the growth and calcification rate of hermatypic coral growth in a natural sea area. The method comprises the following steps: 1, setting a substrate in a coral reef distributed sea area, and mounting a coral reef fixing substrate on the substrate; 2, collecting corallum, weighing the relative weight M0 of the corallum in preset liquid by using a weighing device; adhering the corallum in a coral accommodating substrate to form a connecting body, airing the connecting body, and weighing the relative weight M1 of the connecting body by the weighing method; 3, connecting the coral accommodating substrate with the coral fixing substrate through a connecting piece, and domesticating the coral accommodating substrate and the coral fixing substrate in situ in the coral reef distributed sea area for a while; 4, separating the coral accommodating substrate from the coral reef distributed sea area, weighing the relative weight Mi+1 of the connecting body; 5, connecting the coral accommodating substrate with the coral fixing substrate through the connecting piece, culturing the coral accommodating substrate and the coral fixing substrate for culturing time ti in the coral reef distributed sea area, repeating the step 4, and recording the relative weight Mi+2 of the connecting body after the time ti; and 6, acquiring the growth and calcification rate of the corallum with unit weight in ti time slot through (Mi+2-Mi+1)/(Mi+1-M1+M0)/ti.

The invention belongs to the technical field of preparation of FLP-2 injection solutions, and especially relates to a preparation method of an injection solution for improving weaned piglet intestinal tract development. According to the invention, hGLP-2 is dissolved in a 0.01 mol/L PBS solution with a pH of 7.4; after complete dissolution, the solution is filtrated by a 0.22-micron filter membrane for sterilization, and thus can be stored by refrigeration within a short period. The preparation in which the dissolution of hGLP-2 is realized by the PBS solution causes no stress reaction at all after pig subcutaneous injection; through morphological analysis of intestinal samples, and determination of intestine weight and the intestine weight index, it is found that the feed intake, daily gain, and feed conversion ratio of the piglets during the 2-week test period are improved effectively, which demonstrates that subcutaneous injection of the PBS solution containing hGLP-2 is safe and effective.

The present invention discloses a preparation method for a microencapsulated oral live vaccine of gosling plague. The preparation method comprises: preparing a gosling plague SYG strain into a vaccine half finished product by goose embryo proliferation culture; adding a 5% sucrose and skimmed milk solution after a sterility test is qualified, and uniformly stirring; adding 20-40% porous starch tothe half finished product solution, stirring for 20-40 minutes at a certain temperature, wherein the temperature is controlled to 37 DEG C; mixing the resulting liquid and a 1-2.5% sodium alginate solution, completely stirring, and then carrying out dehydration and drying by a fluidized bed to prepare the microencapsulated vaccine dry powder of the gosling plague, wherein the volume of the resulting liquid is the same as the volume of the sodium alginate solution. The method of the present invention ha characteristics of science, simpleness, stable and reliable production, less loss of vaccine titer. With adopting the vaccine microencapsulation technology, the live vaccine of the gosling plague can be adopted for immunization by the oral route, the stress reaction is reduced, the sustained release function is provided for the live vaccine of the present invention, and the breeder goose in the laying period can use the live vaccine as usual.

The invention belongs to the field of molecular biological technologies, particularly relates to the field of technologies of extracting DNA (Desoxvribose Nucleic Acid) from cattle and goat milk and milk powder, and discloses a method for rapidly and efficiently extracting DNA from cattle and goat liquid milk and milk powder. A Chelex-100 process and a Glassmilk (glass milk) DNA purification technology are perfectly combined and applied to extraction of trace DNA from cattle and goat liquid milk and milk powder for the first time. The collecting quantity of fresh liquid milk can be controlled to be 1-2 ml, the quantity of the milk powder can be controlled to be 1-10 mg, and the extracted DNA is high in concentration, extremely high in purity, and stable without being easily degraded. The extracted DNA can amplify more than 500-1000bp of a long-fragment gene sequence, and the obtained sequence is capable of developing subsequent molecular biology analysis. The method is simple in operation, low in consumption, short in time consumption, high in DNA purity, difficult in degradation, and convenient and practical in sampling; and the problem of difficulty in sampling blood and tissue can be prevented, no animal strsss reaction can be caused, and unnecessary loss caused by other sampling methods to the dairy enterprise and farmers is reduced.

The invention relates to a pig breeding and respiratory syndrome sampling method. A tough cotton sliver is made, the cotton sliver is coated and dipped with inducer, the cotton sliver is fixed on a water drinking device in pig farm colony house, a pig can bite the cotton sliver when drinking water and spittle is adhered on the cotton silver, when the cotton sliver is adhered with spittle, the cotton sliver is taken down and placed into a sample bag, then spittle in the cotton sliver is extruded out, the lower end of the sample bag is opened with an aperture in a tapering way, so that sample is dropwise added into a sample tube along with the aperture, and the sample is refrigerated at 4 DEG C, thus finishing the sampling process. The invention has the advantages that: 1, no restraint person is required, no stress reaction of pig can be induced, no nasal cavity damage on pig can be produced; 2, manpower is saved, operation can be carried out by only one person; 3, the whole process is simple and easy to operate, operation can be carried out only by simple training; 3, one sample is collected in each pigsty and can be on behalf of the whole pigs in the pigsty.

An indoor factory farming Litopenaeus Vannamei water circulation pollution discharge method is provided; the connecting portion between the pool wall and pool bottom is in an arc shape; four bottom corners and four bottom edges of the pool bottom form 1-3% slope with pollution discharge ports, and the pool bottom and the pool wall are smooth; the pollution discharge ports are provided with isolation nets of different number; micropipe oxygenation is arranged on the pool bottom; when the culture pool is filled with water, four water wheels are placed on four corners of the culture pool opening water surface; before pollution discharge, the micropipe oxygenation is partially closed or fully closed, and the water wheels placed on pool opening water surface four corners are opened, so the water circulation can generate eddy currents so as to circulate the pool bottom deposits around the pollution discharge ports, thus discharging the pool bottom deposits outside the pool through the pollution discharge ports. The method is simple and convenient, can save labor and cost; pollution discharge effect can fully cover the pool without leaving blind points, thus thoroughly discharging deposits; basically no deposit can be re-circulated into the culture water in the pollution discharge process; the pollution discharge process is carried out step by step, no stress reaction can be caused on the Litopenaeus Vannamei, and no machinery damage can be caused.

The invention relates to the field of marine fish culturing, and discloses an indoor water segmented marine fish fry breeding method. The method comprises the following steps: (1) fish eggs are hatched; (2) fish fries are cultured. According to the invention, a hatching pool and a nursery pool are separately arranged. Water is purified in advance, and various biological feeds are cultured in advance, such that living conditions in the hatching pool and the nursery pool are regulated, and a relatively stable circulation water condition is provided. No drug such as hormone or antibiotic is needed to be added during the hatching and fish fry breeding processes. Physical and chemical factors such as the temperature, pH value, dissolved oxygen, salinity, and the like of the water in the hatching pool and the nursery pool can remain relatively stable. The water quality is maintained stable, and the fish fries are prevented from having stress responses and drug resistance. A survival rate of the fish fries can reach approximately 14%.

The invention discloses a composition for improving the sub-health state of skin and a preparation method thereof. The composition refers to traditional Chinese medicine famous formulas of 'decoction of four mild drugs' and 'decoction of four drugs', and takes Chinese angelica, bighead atractylodes rhizome, white paeony root, white poria, ligusticum chuanxiong hort, honey-fried licorice root and donkey-hide gelatin as main components; various skin-benefiting nutritional ingredients such as the linseed oil, the collagen oligopeptide powder, the vitamin C, thefructus corni extract, the hyaluronic acid and the glucose are supplemented, so that the effects of invigorating the spleen, replenishing qi, replenishing blood and regulating blood are achieved. Mouse tests prove that by using the composition, the moisture content in mouse skin can be effectively kept, the skin elasticity is increased, no stress response exists, and the effect of improving skin sub-health states such as dark yellow skin color, rough skin and lack of elasticity can be achieved. Besides, the composition is prepared by adopting a special method, effective components in the raw materials are fully extracted and reserved, so that the functions of the components are more stable, and the composition is easy to carry and convenient to use.

The invention discloses a method for evaluating body temperature of sow at a perinatal period and a system thereof. The method includes: obtaining the back surface temperature of the sow, the surface temperature of the eye, the surface temperature of the ear, the temperature of the vulva surface, and the surface temperature of the breast, and performing substitute of the back surface temperature, the eye surface temperature, the ear surface temperature, the vulvar surface temperature, and the breast surface temperature into an evaluation mathematical model to obtain the sow's body temperature. By measuring the surface temperature of each part of the sow, a temperature value is obtained by the above measurement, and a body temperature value of the sow is obtained through the evaluating the evaluation mathematical model. The system includes a temperature acquisition device and a body temperature calculation device. Through a non-invasive measurement method, the sow's stress response cannot be caused under the premise of correctly reflecting the sow body temperature. The system is conducive to pig farming operations.

The invention discloses a six-channel movable intelligent respiratory-metabolic measuring device for cattle. The respiratory-metabolic measuring device comprises a data analysis device and a respiratory-metabolic device, and the respiratory-metabolic device comprises a weighing device, a feed weighing device, an ear-mark identifying device and a gas collection device; the data analysis device comprises an industrial personal computer and a gas analysis device; the gas collection device is connected to the gas analysis device, and the gas analysis device is further connected to the industrial personal computer; the weighing device, the feed weighing device and the ear-mark identifying device are connected with the industrial personal computer separately. According to the six-channel movable intelligent respiratory-metabolic measuring device for the cattle, radio-frequency electronic ear marks are adopted to identify and mark animals being measured, the ear-mark number, weight, feed amount and discharged CH4 and CO2 of each cattle are measured in the feeding process of the animals, the experimental cardinal number is large, and no stress response occurs; recording can be conducted in a reciprocating and circulating mode for a long term to create a record for each animal. The measuring device is convenient to disassemble and assemble, easy to convey, super high in anti-shake performance, and good in stability and data repeatability under the condition of an open pasture, and can be continuously used for a long term.

The invention relates to channel type pasture spraying disinfecting and sterilizing equipment. The channel type pasture spraying disinfecting and sterilizing equipment consists of spraying assemblies, a pesticide groove, fences, an automatic pesticide adding box, a disinfection channel main machine, a water inlet pipe and pesticide outlet pipes. The four groups of spraying assemblies are parallel to one another and are uniformly erected on a bottom pesticide tank in the length direction of the channel, the fences are arranged on the left and right sides of the four groups of spraying assemblies, the automatic pesticide adding box and the disinfection channel main machine are arranged on one side outside the channel and are effectively connected through a line, and the water inlet pipe is arranged at one end part of the automatic pesticide adding box, one ends of the four groups of the pesticide outlet pipes are connected with the disinfection channel main machine, and the other ends are respectively connected with the four groups of the spraying assemblies. In the working process, when cattle and sheep in a pasture pass through the disinfection channel, the disinfection channel main machine starts a working mode, liquid pesticide is conveyed to all nozzles of the four groups of the spraying assemblies through the four pesticide outlet pipes and sprayed to the bodies of the cattle and the sheep, besides, the feet of the cattle and the sheep pass through the pesticide tank to complete the working procedures of dust removal, soaking, water absorption and the like, and one-time disinfecting and sterilizing are completed.

The invention provides a preparation method for preparing a Muscovy duck hepatitis virus recombinant chicken colony stimulating factor adjuvant inactivated vaccine by means of a Muscovy duck hepatitis virus GS14. The vaccine is prepared by taking the Muscovy duck hepatitis virus GS14 as immunogen and taking a recombinant chicken colony stimulating factor as an adjuvant. The Muscovy duck hepatitis virus GS14 is preserved in China Center for Type Culture Collection on April 27, 2016, and the preservation number is CCTCC No:V201623. The inactivated vaccine has the advantages that the vaccine can be intramuscularly injected or subcutaneously injected in use and can be rapidly absorbed, no granuloma is formed, a stress reaction to young Muscovy ducks basically does not exist, and the vaccine has better safety compared with propolis and oil adjuvant vaccines. When the vaccine is used for prevention, the protection rate is 90% or above, and the protection period lasts for three months; the recombinant chicken colony stimulating factor, serving as the immunologic adjuvant, in the vaccine has the effect of stimulating immune cell development of organisms and can non-specifically enhance the immune function of the organisms.

The invention provides an automatic feeding device and method for low-day-age young pigeons. The automatic feeding device for the low-day-age young pigeons comprises an air compressor, a feed discharging barrel, an air path switching valve and a feeding gun; the air compressor is communicated with the feed discharging barrel through a first air path branch pipe, a first pressure regulating valve is arranged on the first air path branch pipe, and the feed discharging barrel is communicated with the feeding gun; and the air compressor is communicated with the air path switching valve through a second air path branch pipe, the air path switching valve is communicated with the feeding gun, and a second pressure regulating valve is arranged on the second air path branch pipe. According to the automatic feeding device for the low-day-age young pigeons, the discharging amount and the discharging speed needed by the young pigeons can be self-adapted according to the different-day-age young pigeons, the accurate given feeding amount of the young pigeons is finally achieved, the feeding and discharging speed can be adjusted, and the young pigeons cannot generate stress reactions after being fed through the automatic feeding device for the low-day-age young pigeons.

The invention provides a nursing and fattening large-fence pig movement monitoring method and system, and the method comprises the steps: collecting the images of pigs through a camera, and enabling the camera to be located right above a pig fence; processing the images of the pigs; analyzing the processed image of the pig, and obtaining the motion condition of the pig according to an analysis result. According to the method, the movement condition of the pig is obtained by collecting the image of the pig and processing and analyzing the image of the pig, compared with the prior art, equipment does not need to be worn on the pig, the stress reaction of the pig cannot be caused, the cost is saved, and application is more convenient.

The invention provides an application of tea saponin in killing of aquatic harmful organisms in Apostichopus japonicas breeding, and belongs to the technical field of aquaculture. The application of tea saponin in killing of aquatic harmful organisms in Apostichopus japonicas breeding solves the problems of large extra consumption of manpower, material resources and time cost, influence on normalproduction planning and easy stimuli and physical damage to young Apostichopus japonicas in the operation process of a manual hand picking method in the prior art, and also solves the problems of complex medicine formula, long medicated bath time and complex medicated bath post-treatment process in the killing process of Chinese herbal medicine compound treatment. The tea saponin has the characteristics of high killing efficiency, thorough effect, no extra labor or time cost, no side effects on Apostichopus japonicas, and easiness in large-scale promotion and application.