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Preparation method of injection solution for improving weaned piglet intestinal tract development

A technology for intestinal development and weaning piglets, which can be applied to medical preparations containing active ingredients, pharmaceutical formulas, inorganic non-active ingredients, etc. , the effect of convenient use

Inactive Publication Date: 2013-03-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] The disadvantage is that the movement of the animal subjects needs to be restricted, and an operation is required to install a venous cannula; the general experimental conditions are difficult to meet its strict requirements; it has obvious application limitations

Method used

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Embodiment Construction

[0010] The following examples can enable those skilled in the art to understand the present invention more comprehensively, but do not limit the present invention in any way.

[0011] The preparation method of the injection solution for improving the intestinal development of weaned piglets given in this example is characterized in that: hGLP-2 freeze-dried powder is dissolved in a 0.01 mol / L phosphate buffer solution (PBS solution, purchased from the market) with a pH of 7.4. PBS powder with a pH of 7.4, 0.01 M and ultrapure water prepared from the commercially available product), after being completely dissolved, filter through a 0.22 μm filter membrane to sterilize. It can be used directly after the preparation is completed, and it can be stored in refrigerator for short-term use, otherwise it can be stored at -20°C after aliquoting and cannot be frozen and thawed repeatedly.

[0012] A 0.01 mol / L phosphate buffer solution with a pH of 7.4 can also be prepared by yourself. ...

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Abstract

The invention belongs to the technical field of preparation of FLP-2 injection solutions, and especially relates to a preparation method of an injection solution for improving weaned piglet intestinal tract development. According to the invention, hGLP-2 is dissolved in a 0.01 mol / L PBS solution with a pH of 7.4; after complete dissolution, the solution is filtrated by a 0.22-micron filter membrane for sterilization, and thus can be stored by refrigeration within a short period. The preparation in which the dissolution of hGLP-2 is realized by the PBS solution causes no stress reaction at all after pig subcutaneous injection; through morphological analysis of intestinal samples, and determination of intestine weight and the intestine weight index, it is found that the feed intake, daily gain, and feed conversion ratio of the piglets during the 2-week test period are improved effectively, which demonstrates that subcutaneous injection of the PBS solution containing hGLP-2 is safe and effective.

Description

technical field [0001] The invention belongs to the technical field of preparation of GLP-2 injection solution, in particular to a method for preparing and using an injection solution for improving intestinal development of weaned piglets. Background technique [0002] Newborn piglets receiving total parenteral nutrition (TPN) after surgery were given exogenous supplementation of glucagon-like peptide-2 (GLP-2) by jugular vein infusion at a dose of 500 pmol kg –1 h –1 , continuous infusion 7d. GLP-2 solutions were prepared by dissolving humanGLP-2 (hGLP-2) in a sterile solution of 0.9% NaCl containing 0.1% human serum albumin (Cottrell et al., 2005); similarly, Burrin et al. (2005 and 2008 ) test using continuous intravenous injection at 0.5 ml.kg -1 .h -1 Supplementation of exogenous GLP-2 to TPN piglets. The preparation of GLP-2 solution is hGLP-2 1-33 Dissolved in 0.45g / L NaCl solution containing 0.5% human serum albumin, so that the final concentrations were 0.8,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K38/26A61K47/02A61P1/00
Inventor 贾刚邓秋红蒋义余长松
Owner SICHUAN AGRI UNIV
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