Method for separating and screening anti-tumor active endophytic fungi of schisandra

An endophytic fungus, separation and screening technology, applied in the field of microorganisms, can solve the problems of increasing the volume of goods, unable to meet the demand of the market, and reducing the output of wild Schisandra chinensis, and achieve the effect of improving the success rate and efficiency.

Inactive Publication Date: 2011-05-18
TIANJIN POLYTECHNIC UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

As early as 1987, Schisandra chinensis was included in the catalog of "Regulations on the Protection and Management of Wild Medicinal Resources". However, according to the survey, the production of wild Schisandra chinensis decreased year after year from 2000 to 2006, and Schisandra chinensis is a traditional commodity exported by my country. It is exported in large quantities to Japan, In South Korea, Singapore and other countries, the demand for goods has increased year after year, and the existing wild resources of Schisandra chinensis can no longer meet the market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The stem tissue of Schisandra chinensis was collected from Shengli Nursery in Huanren, Liaoning, and the surface was sterilized according to the following procedure: the stem was cut into 2.0 cm small pieces, rinsed with sterile water, quickly rinsed in 75% ethanol for about 1 min, rinsed with sterile water for 3 times, and used Disinfect with 0.1% mercuric chloride for 8 minutes, and finally rinse with sterile water for 3 times, put it on a sterilized filter paper sheet, inoculate it on a PDA plate medium after the water is blotted dry, and incubate at 28°C for 3 to 5 days. The stem tissue on which the strain grows.

[0021] The aseptically tested stem tissue was cut into small pieces of about 0.5 cm × 0.5 cm under the ultra-clean workbench, and then the small pieces were planted on a PDA medium plate and cultured in a 28°C incubator. When hyphae obviously grew around the sample, the tip mycelia picking method was used to pick colonies with different shapes, and after ...

Embodiment 2

[0027] Take Schisandra cotyledons, and disinfect the surface according to the following procedures: rinse with sterile water, quickly rinse in 75% ethanol for about 1 min, rinse with sterile water for 3 times, then disinfect with 0.1% mercury liter for 5 min, and finally rinse with sterile water for 3 times, put On the sterilized filter paper sheet, inoculate it on the PDA plate culture medium after the water is blotted dry, culture at 28°C for 3-5 days, and select the leaf tissue without any bacteria growing on the surface.

[0028] The leaf tissue tested for sterility was cut into small pieces of about 0.5 cm × 0.5 cm under ultra-clean bench conditions, and then the small pieces were planted on PDA medium plates and cultured in an incubator at 28°C. When hyphae obviously grew around the sample, the tip mycelia picking method was used to pick colonies with different shapes, and after purification, they were transferred to PDA slant medium for future use.

[0029] The above-me...

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PUM

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Abstract

The invention discloses a method for separating and screening endophytic fungi of schisandra, and belongs to the technical field of microbes. The method comprises the steps of preparation of schisandra tissues, surface disinfection and sterile detection, separation and culture of the endophytic fungi of the schisandra tissues, preparation of practical fermentation medium, culture of the endophytic fungi, screening of strains and the like. The invention provides the effective strain separating and screening method for extracting anti-tumor active substances from the schisandra plant tissues by substituting a microbial fermentation method.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for isolating and screening endophytic fungi capable of producing anti-tumor metabolites from schisandra tissues. technical background [0002] Schisandra Chinensis Baill. and S. sphenanthera Stapf. recorded in the Pharmacopoeia of the People's Republic of China have the effects of astringent and astringent, nourishing qi and promoting body fluid, tonifying kidney and calming heart. In recent years, studies have found that the rhizomes of Schisandra plants have good anti-tumor and anti-HIV activities, causing the research on Schisandra plants to once again attract widespread attention at home and abroad, which makes the wild resources of Schisandra plants face another challenge. As early as 1987, Schisandra chinensis was included in the catalog of "Regulations on the Protection and Management of Wild Medicinal Resources". However, according to the survey, the production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 郭文娟王翔张慧春闻婷婷李洛
Owner TIANJIN POLYTECHNIC UNIV
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