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Application of blood plasma miR-208a in early marker for diagnosis of acute myocardial infarction

A technology of mir-208a, acute myocardial infarction, applied in the field of medical molecular biology

Inactive Publication Date: 2011-07-27
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

However, for the diagnosis of patients with early myocardial infarction (within 3 hours of chest pain), there is currently no good specific marker available, so finding markers that can be used for early diagnosis of acute myocardial infarction has broad market prospects

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  • Application of blood plasma miR-208a in early marker for diagnosis of acute myocardial infarction
  • Application of blood plasma miR-208a in early marker for diagnosis of acute myocardial infarction
  • Application of blood plasma miR-208a in early marker for diagnosis of acute myocardial infarction

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Embodiment Construction

[0063] The technical solution of the present invention will be described in detail below with reference to the accompanying drawings.

[0064] Implementation method of plasma miR-208a in the early diagnosis of acute myocardial infarction:

[0065] (1) Plasma collection:

[0066] 1ml of blood was drawn from the patient and centrifuged in two steps at 4°C. Finally, the upper plasma was collected in a RNase / DNase-free centrifuge tube.

[0067] (2) Extraction of plasma total RNA:

[0068] Take 200μl of plasma and extract total RNA from plasma by optimized plasma RNA extraction method, and add cel-miR-39 as the internal control of the system during the extraction process.

[0069] (3) Real-time PCR reaction:

[0070] Use the miRNA detection kits (including reverse transcription kits, specific miRNA primers and probes, fluorescence quantitative detection kits, etc.) from Applied Biology to detect miR-208a and cel-miR-39 in plasma and other reference miRNAs ( For example, the level of miR-16, e...

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Abstract

The invention discloses the new purpose of blood plasma miR-208a in an early marker for the diagnosis of acute myocardial infarction. The miRNA only has 18 to 25 nucleotide, and a mature body of the miRNA stably exists in cytoplasm, therefore, after the occurrence of the acute myocardial infarction, the miRNA is released into circulating blood earlier than structural protein substances (such as troponin), the raise of level can be detected at the early stage of myocardial infarction, and the blood plasma miR-208a can be used as the early marker for the diagnosis of acute myocardial infarction. With regard to the diagnoses to patients at the early stage of myocardial infarction (within three hours of stethalgia), good specific markers are currently unavailable, therefore, the blood plasma miR-208a has broad market prospect in finding out the marker applicable to the early diagnosis of acute myocardial infarction.

Description

【Technical Field】 [0001] The present invention relates to the technical field of medical molecular biology, and more specifically, to the new use of plasma miR-208a as an early marker for the diagnosis of acute myocardial infarction. 【Background technique】 [0002] MicroRNA (miRNA) is a type of endogenous non-coding small RNAs with only 18-25 nucleotides, which are widely present in various organisms. miRNA binds to the 3'untranslated region (3'-UTR) of target gene mRNA, degrades or inhibits mRNA translation, regulates the expression of target genes, and participates in regulating various biological functions. In 1993, Ambros first discovered a non-coding single-stranded small molecule RNA-lin4 in C. elegans, which has the function of regulating the developmental phase of C. elegans. After nearly 20 years of scientific research, 721 miRNAs (Sanger miRbase 14.0) have been discovered in the human body. These miRNAs play an important regulatory role in many life activities of the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 荆清秦永文王国坤朱嘉琦
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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