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Active extracts of rosa roxburghii tratt fruit, and preparation method, detection method and application thereof

A detection method and extract technology, applied in the field of active extracts from active parts of triterpenoids and flavonoids-catechins of Rosa roxburghii and its preparation, active extracts from Rosa roxburghii, active extracts from effective parts of triterpenes from Rosa roxburghii , can solve the problems of extraction, purification, analysis, detection and other researches that are still blank

Active Publication Date: 2011-08-10
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the extraction, refinement, analysis and detection of the anti-tumor effective parts in Rosa roxburghii is still blank so far.

Method used

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  • Active extracts of rosa roxburghii tratt fruit, and preparation method, detection method and application thereof
  • Active extracts of rosa roxburghii tratt fruit, and preparation method, detection method and application thereof
  • Active extracts of rosa roxburghii tratt fruit, and preparation method, detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] Embodiment 1: the preparation of Rosa roxburghii fruit active extract: get Rosa roxburghii fresh fruit 1kg, reflux extraction 2 times with 80% ethanol, each 2 hours, the amount of alcohol added is 10 times of raw material weight, filters, merges filtrate, reclaims Ethanol until there is no alcohol smell, add water to dilute the extract to a relative density of 1.15, extract three times with ethyl acetate, the ratio of ethyl acetate to water is 1:1, combine the ethyl acetate layers, recover ethyl acetate, add water and sonicate Or heat to disperse, filter with suction, wash the precipitate with water for 3 times, combine the filtrate, and vacuum-dry the filtrate to obtain active extract B, and vacuum-dry the precipitate to obtain active extract A.

Embodiment 2

[0111] Embodiment 2: the preparation of Rosa roxburghii fruit active extract: get Rosa roxburghii fruit 1kg, reflux extraction with 95% ethanol for 3 hours, the amount of alcohol added is 12 times of raw material weight, filters, merges filtrate, reclaims ethanol to no alcohol smell , add water to the extractant with a relative density of 1.25, extract 4 times with ethyl acetate, the ratio of ethyl acetate to water is 0.5:1, combine the ethyl acetate layers, recover ethyl acetate, add water and ultrasonically or heat to disperse, Suction filtration, washing the precipitate with water for 4 times, combining the filtrates, and drying the filtrate and the precipitates in a microwave vacuum to obtain active extract B and active extract A respectively.

Embodiment 3

[0112] Embodiment 3: the preparation of Rosa roxburghii fruit active extract: get Rosa roxburghii fresh fruit 1kg, reflux extraction with 70% methanol 3 times, each 1.5 hours, the amount of alcohol added is 6 times of raw material weight, filters, merges filtrate, reclaims Methanol until there is no alcohol smell, add water to dilute the extract to a relative density of 1.1, extract 4 times with n-butanol, the ratio of n-butanol to water is 1:1, combine the n-butanol layers, recover n-butanol, add water and sonicate Or heat to disperse, filter with suction, wash the precipitate with water for 3 times, combine the filtrate, and dry the filtrate and the precipitate under reduced pressure and vacuum to obtain active extract B and active extract A respectively.

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PUM

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Abstract

The invention discloses active extracts of rosa roxburghii tratt fruit, and a preparation method, a detection method and application thereof. The preparation method comprises the following steps of: extracting rosa roxburghii tratt fruit by conventional processes; filtering to remove rosa roxburghii tratt residues; concentrating the filtered solution at the reduced pressure; extracting with an organic solvent; recovering the organic solvent; adding water for dispersing; filtering; washing with water for depositing; mixing the filtered solutions; and respectively drying the filtered solution and the deposit to respectively obtain an active extract B and an active extract A. The preparation method provided by the invention solves the problems of extraction, enrichment and refinement of effective active components in the rosa roxburghii tratt, and industrial production is easy to realize by the preparation process; the detection method provided by the invention can effectively ensure thequality of the active extracts; and the obtained active extracts have obvious anti-malignant tumor activity, have synergistic action with clinical anti-cancer drugs, have better alpha-glucosidase inhibition activity, can be used for preparing drugs and adjuvant drugs for treating malignant tumors or used as health food additives, can also be used for preparing drugs and health products for preventing or treating diabetes or used as food additives, and have higher application values and good application prospects.

Description

technical field [0001] The present invention relates to the active extract of Rosa roxburghii, especially the active extract of triterpenoid active parts of Rosa roxburghii and the active extract of triterpenes and flavonoid (+)-catechin effective parts of Rosa roxburghii and its preparation method, detection method and The application belongs to the technical field of traditional Chinese medicine or natural medicine. Background technique [0002] Malignant tumor (cancer) is a disease that seriously endangers human health. According to statistics, in 2007, there were about 12 million new cancer patients in the world, 7.6 million people died of cancer, and about 20,000 people died of cancer every day. In addition, the incidence of cancer continues to rise, and it is estimated that there will be 15 million new cancer patients worldwide in 2020. Lung cancer, liver cancer, stomach cancer, esophageal cancer, colon cancer, breast cancer, cervical cancer and nasopharyngeal cancer...

Claims

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Application Information

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IPC IPC(8): A61K36/738A23L1/30G01N21/78G01N21/31G01N30/36A61P3/10A61P35/00A61K131/00
Inventor 杨小生马琳梁冰余丽梅朱海燕李淑芳
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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