Method for realizing tissue culture on asparagus

A technique for tissue culture and asparagus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of limited tissue culture effect, low rooting rate of asparagus tissue culture seedlings, low transplanting survival rate, etc.

Inactive Publication Date: 2011-08-24
LIANYUNGANG ACAD OF AGRI SCI
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, the problems of low rooting rate and transplanting survival rate of asparagus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1. A method for asparagus tissue culture, the steps are as follows:

[0045] (1) Select healthy plants in the field, and use alcohol-wiped scissors to cut off 4cm long branches (with stem tips); after retrieving, cut off a section at the cut, and use 75% alcohol by volume to add mass-volume The aqueous solution of sodium dodecylbenzenesulfonate with a percentage concentration of 0.5% was soaked in ultrasonic for 30 seconds, then added with a mass-volume percentage concentration of 0.1% mercuric chloride aqueous solution, shaken and soaked for 5 minutes (shaking), washed with sterile water for 4 times, taken out and placed in a on sterilized absorbent paper;

[0046] (2) Cut off the sterilized asparagus shoot tips under sterile conditions, and inoculate them into asparagus proliferation medium; firstly cultivate at 18°C ​​in the dark for 3 days, then transfer to 1500Lx25°C for 5 days and then adjust the light to 2000Lx It can be expanded every 20 days thereafte...

Example Embodiment

[0078] Example 2. A method for asparagus tissue culture, the steps are as follows:

[0079] (1) Select healthy plants in the field, and use alcohol-wiped scissors to cut off 5cm long branches (with stem tips); after retrieving, cut off a section at the cut, and use a volume percentage concentration of 75% alcohol plus mass-volume The aqueous solution of sodium dodecylbenzenesulfonate with a percentage concentration of 0.5% was soaked in ultrasonic for 30 seconds, then added with a mass-volume percentage concentration of 0.1% mercuric chloride aqueous solution, shaken and soaked for 5 minutes (shaking), washed with sterile water for 4 times, taken out and placed in a on sterilized absorbent paper;

[0080] (2) Cut off the sterilized asparagus shoot tips under sterile conditions, and inoculate them into asparagus proliferation medium; firstly cultivate at 18°C ​​in the dark for 3 days, then transfer to 1500Lx25°C for 5 days and then adjust the light to 2000Lx After that, it ca...

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PUM

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Abstract

The invention relates to a method for realizing tissue culture on asparagus. The method is characterized by comprising the following steps of: selecting healthy asparagus plants in a field, cutting a long branch provided with stem tips; sterilizing and then inoculating the long branch propagation culture medium; firstly culturing in a dark place, then carrying out lighting culture and propagation; transplanting an asparagus single strong seedling obtained by propagation into a rooting culture medium to be cultured, after a root is grown, cutting the root, and then transplanting the strong seedling into a rooting culture medium II to be cultured; and finally dipping the root in solution containing bacillus mucilaginosus and streptomyces microflavus Beijing subspecies, transplanting the root into a pot filled with sandy soil, and culturing for 20-30 days under the condition of natural illumination, thus a survival asparagus seedling is obtained. In the invention, a two-steps rooting method is adopted, and activated carbon with appropriate concentration is added to improve the rooting rate of asparagus; and in the seedling transplanting process, the root is dipped the solution containing bacillus mucilaginosus and streptomyces microflavus Beijing subspecies, thus the survival rate of transplanting is improved.

Description

technical field [0001] The invention relates to a method for cultivating plants, in particular to a method for culturing asparagus tissue. Background technique [0002] Asparagus, also known as asparagus and asparagus, is internationally recognized as the "king of vegetables". Because asparagus is rich in nutrients and has good health care and medical effects, the number of people who like to eat asparagus is increasing year by year. The demand for asparagus products is also increasing day by day, and has formed a long-term shortage of supply. [0003] The high-quality F1 generation asparagus seeds used in production have been imported from abroad, which is expensive. The use of tissue culture technology for asexual rapid propagation of asparagus can not only solve the degradation of asparagus species, but also cultivate a large number of high-quality asparagus seedlings to meet the needs of production; because the output of male asparagus plants is 20% to 30% higher than t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈振泰潘美红缪美华薛萍杨海峰孙桂乐周翔
Owner LIANYUNGANG ACAD OF AGRI SCI
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