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Method for realizing tissue culture on asparagus

A technique for tissue culture and asparagus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of limited tissue culture effect, low rooting rate of asparagus tissue culture seedlings, low transplanting survival rate, etc.

Inactive Publication Date: 2011-08-24
LIANYUNGANG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problems of low rooting rate and transplanting survival rate of asparagus tissue culture seedlings are common, which greatly limits the effect of tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. A method for asparagus tissue culture, the steps are as follows:

[0045] (1) Select a healthy plant in the field, and cut off a 4cm long branch (with stem tip) with scissors wiped with alcohol; after taking it back, cut off a section at the cut, and add mass-volume with 75% alcohol by volume Ultrasonic immersion in 0.5% sodium dodecylbenzenesulfonate aqueous solution for 30 seconds, then add 0.1% mass-volume mercuric acid aqueous solution, shake and soak for 5 minutes (shake), wash with sterile water 4 times, take it out and place it in on sterilized absorbent paper;

[0046] (2) Cut off the sterilized asparagus stem tip under aseptic conditions and inoculate it into the asparagus proliferation medium; culture at 18°C ​​for 3 days in the dark, then transfer to 1500Lx25°C for 5 days and then adjust the light to 2000Lx Thereafter, it can be multiplied every 20 days;

[0047] (3) The asparagus single strong seedlings obtained from the multiplication are tran...

Embodiment 2

[0078] Example 2. A method for asparagus tissue culture, the steps are as follows:

[0079] (1) Select healthy plants in the field, and cut off 5cm long branches (with stem tips) with scissors wiped with alcohol; after retrieval, cut off a section at the cut, and add mass-volume with 75% alcohol by volume Ultrasonic immersion in 0.5% sodium dodecylbenzenesulfonate aqueous solution for 30 seconds, then add 0.1% mass-volume mercuric acid aqueous solution, shake and soak for 5 minutes (shake), wash with sterile water 4 times, take it out and place it in on sterilized absorbent paper;

[0080] (2) Cut off the sterilized asparagus stem tip under aseptic conditions and inoculate it into the asparagus proliferation medium; culture at 18°C ​​for 3 days in the dark, then transfer to 1500Lx25°C for 5 days and then adjust the light to 2000Lx Subsequent multiplication can be done every 30 days;

[0081] (3) The asparagus single strong seedlings obtained from the multiplication are tran...

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PUM

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Abstract

The invention relates to a method for realizing tissue culture on asparagus. The method is characterized by comprising the following steps of: selecting healthy asparagus plants in a field, cutting a long branch provided with stem tips; sterilizing and then inoculating the long branch propagation culture medium; firstly culturing in a dark place, then carrying out lighting culture and propagation; transplanting an asparagus single strong seedling obtained by propagation into a rooting culture medium to be cultured, after a root is grown, cutting the root, and then transplanting the strong seedling into a rooting culture medium II to be cultured; and finally dipping the root in solution containing bacillus mucilaginosus and streptomyces microflavus Beijing subspecies, transplanting the root into a pot filled with sandy soil, and culturing for 20-30 days under the condition of natural illumination, thus a survival asparagus seedling is obtained. In the invention, a two-steps rooting method is adopted, and activated carbon with appropriate concentration is added to improve the rooting rate of asparagus; and in the seedling transplanting process, the root is dipped the solution containing bacillus mucilaginosus and streptomyces microflavus Beijing subspecies, thus the survival rate of transplanting is improved.

Description

technical field [0001] The invention relates to a method for cultivating plants, in particular to a method for culturing asparagus tissue. Background technique [0002] Asparagus, also known as asparagus and asparagus, is internationally recognized as the "king of vegetables". Because asparagus is rich in nutrients and has good health care and medical effects, the number of people who like to eat asparagus is increasing year by year. The demand for asparagus products is also increasing day by day, and has formed a long-term shortage of supply. [0003] The high-quality F1 generation asparagus seeds used in production have been imported from abroad, which is expensive. The use of tissue culture technology for asexual rapid propagation of asparagus can not only solve the degradation of asparagus species, but also cultivate a large number of high-quality asparagus seedlings to meet the needs of production; because the output of male asparagus plants is 20% to 30% higher than t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈振泰潘美红缪美华薛萍杨海峰孙桂乐周翔
Owner LIANYUNGANG ACAD OF AGRI SCI
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