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Micropropagation method for castanopsis hystrix twigs

A technique of twigs and micropropagation, which is applied in the field of plant cultivation, can solve the problems of unfavorable preservation and development of red cones, cannot satisfy a large number of seedlings, and unstable cutting survival, so as to improve the survival rate of cuttings, shorten the rooting time of cuttings, shorten The effect of collection interval

Inactive Publication Date: 2012-09-05
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of awareness of the protection of native tree species and the drive of economic interests, the area of ​​​​the natural forest of red cones is decreasing day by day, and the red cones are strong in different years, and there are almost no harvests in small years, which is not conducive to red cones Cone Conservation and Development
[0004] At present, the cutting propagation research technology of red cone is limited to the conventional cuttage cutting of mother tree cuttings and field planting cuttings of mother plants to promote germination. It is easy to be limited by meteorological conditions, the quality of cuttings is low, and the survival of cuttings is unstable, which cannot meet the needs of a large number of production. use seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]From November to December every year, select excellent plants of red cones that are more than 20 years old, with straight trunks, well-proportioned crowns, strong growth and no pests and diseases, collect fruits, and dry them in the shade indoors until the seed shells dry and crack, until the seeds fall off. Soak the seeds in clean water, change the water every day, take out the seeds after 7 days, rinse them with clean water, sow them on a clean wet sand bed in the shade shed, lightly press the seeds with a wooden board to sink into the sand, and then cover the seeds with sand. It is advisable not to see the seeds, and finally sprinkle with water. In March of the following year, dig out the sprouts that have grown roots and buds but have not yet unfolded the true leaves from the sand bed, and cut off the main root with a sharp knife from a distance of 1.5cm from the seeds, and then transplant them into the shade shed with a medium specification of 8× In a 10cm container...

Embodiment 2

[0037] From November to December every year, select excellent plants of red cones that are more than 20 years old, with straight trunks, well-proportioned crowns, strong growth and no pests and diseases, collect fruits, and dry them in the shade indoors until the seed shells dry and crack, until the seeds fall off. Soak the seeds in clean water, change the water every day, take out the seeds after 7 days, rinse them with clean water, sow them on a clean wet sand bed in the shade shed, lightly press the seeds with a wooden board to sink into the sand, and then cover the seeds with sand. It is advisable not to see the seeds, and finally sprinkle with water. In April of the following year, dig out the sprouts that have grown roots and buds but the true leaves have not yet unfolded from the sand bed, and cut off the main root with a sharp knife from a distance of 2cm from the seeds, and then transplant them into the shade shed with a substrate with a size of 10×12cm. In a containe...

Embodiment 3

[0039] From November to December every year, select excellent plants of red cones that are more than 20 years old, with straight trunks, well-proportioned crowns, strong growth and no pests and diseases, collect fruits, and dry them in the shade indoors until the seed shells dry and crack, until the seeds fall off. Soak the seeds in clean water, change the water every day, take out the seeds after 7 days, rinse them with clean water, sow them on a clean wet sand bed in the shade shed, lightly press the seeds with a wooden board to sink into the sand, and then cover the seeds with sand. It is advisable not to see the seeds, and finally sprinkle with water. In May of the following year, dig out the sprouts that have grown roots and buds but the true leaves have not yet unfolded from the sand bed, and cut off the main root with a sharp knife from a distance of 3 cm from the seeds, and then transplant them into the shade shed with a substrate with a size of 12 × 14 cm. In a contai...

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Abstract

The invention relates to a micropropagation method for castanopsis hystrix twigs. The micropropagation method comprises the following steps: conventionally accelerating germination of high-quality seeds of castanopsis hystrix of more than 20 years old, and transplanting into a container cup for seedling cultivation; transplanting seedlings capable of receiving complete illumination onto an elevated seedling culturing bed containing base materials in a plastic greenhouse for seedling cultivation; cutting tops to promote sprouting, collecting lateral shoots, and transplanting into a container cup containing base materials in a cuttage way; and placing the container cup on the elevated seedling culturing bed in the plastic greenhouse for seedling cultivation to obtain container cuttage seedlings of castanopsis hystrix. By adopting the elevated seedling culturing bed and the seedling culturing base materials taking yellow soil as the main part to plant parent seedlings, water and nutrition conditions can be favorably controlled, and high-quality cut shoots can be cultured; meanwhile, when an air temperature and humidity regulator of the plastic greenhouse is used to strictly control the relative humidity and temperature of the air in the seedling culturing bed, the cuttage survival rate of castanopsis hystrix reaches more than 90-97%, and each parent seedling can produce 20-40 shoots. The method for culturing cuttage seedlings of castanopsis hystrix is convenient to operate, has low production cost, and can be used for rapidly culturing a large amount of high-quality asexual seedlings for forest planting, thereby having favorable market application prospects.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a micropropagation method for red cone twigs. Background technique [0002] Red cone (Castanopsis hystrix) is an evergreen tree of the Fagaceae Castanopsis genus, distributed in the south of Guangxi, Guangdong, Hainan, Yunnan, Guizhou, Hunan, Jiangxi, Fujian and other provinces, Medog County in Tibet, Vietnam, Myanmar, India There are also distributions. The central distribution area is in the hilly area below 700m above sea level in southern Guangxi. It grows fast, has strong germination, dense branches and leaves, is more resistant to shade, and has good mixed growth performance. It is the first choice for mixed afforestation with pine, fir or other broad-leaved trees in the hilly areas of South China. Multi-purpose tree species. Red cone material is excellent, the wood is hard and corrosion-resistant, the heartwood is large, brownish red, the sapwood i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G23/00A01G9/10
CPCY02A40/22Y02P60/40
Inventor 郝海坤黄开勇黄志玲彭玉华曹艳云姜英
Owner GUANGXI FORESTRY RES INST
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