Three-dimensional super-resolution focusing method and device based on transparent medium pellet

A technology of transparent medium and focusing method, applied in optical elements, optics, instruments, etc., can solve problems such as limitations, and achieve the effect of simple structure, easy realization principle, and small three-dimensional focusing volume.

Inactive Publication Date: 2012-11-14
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

However, due to the dependence of secondary excitation on the intensity of light illumination, two-photon excitation is limited to a small focal volume, and the spatial resolution of two-photon fluorescence techniques based on conventional optics is still limited by the diffraction limit.

Method used

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  • Three-dimensional super-resolution focusing method and device based on transparent medium pellet
  • Three-dimensional super-resolution focusing method and device based on transparent medium pellet
  • Three-dimensional super-resolution focusing method and device based on transparent medium pellet

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Embodiment Construction

[0033] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, but the present invention is not limited thereto.

[0034] Such as figure 2 As shown, a device for realizing three-dimensional super-resolution focusing includes: a light source 1 , a microscope objective lens 4 , a cover glass 3 and a transparent medium ball 2 . The microscope objective lens 4, the transparent medium ball 2 and the cover glass 3 are all located on the optical path of the light beam emitted by the light source 1. The transparent medium ball 2 is a transparent medium ball in the order of microns, with a size of 1-10um and a refractive index of 1.4 ~2, the transparent medium ball 2 is located near the focal plane of the microscope objective lens 4, and is fixed by the cover glass 3, and an ultra-small focused spot can be obtained at the exit end of the transparent medium ball 2, which is used to irradiate or detect the sample 5 to obtai...

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PUM

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Abstract

The invention discloses a three-dimensional super-resolution focusing method and device based on transparent medium pellet. The method comprises the steps of: emitting a radial polarized light beam to a micron-grade transparent medium pellet to focus to obtain a focused light spot at the exit end of the transparent medium pellet. The device orderly comprises a device for generating the radial polarized light beam, a micro-objective, the micron-grade transparent medium pellet and a cover glass for fixing the micron-grade transparent medium pellet, wherein the radial polarized light beam is primarily focused by the micro-objective and then further focused by the micron-grade transparent medium pellet to obtain the focused light spot. In the invention, a single light beam is used as a systemworking light beam, which saves system cost and increases practical applicability; simultaneously, the longitudinal axis length of the focused light spot is reduced to be less than 0.17 lambda, the quality of the light beam is increased, and the transversal size of the focused light spot can be lower than the diffraction limit of the system; in particular, the volume of the focused light spot is small, which is favorable for actual application.

Description

technical field [0001] The invention belongs to the field of optical metrology and super-resolution imaging, and in particular relates to a three-dimensional super-resolution focusing method and device for realizing an ultra-small focusing field. Background technique [0002] With the development of current technology, the objects that come into contact with tend to be miniaturized, and the objects that need to be manufactured or observed are increasingly beyond the range that can be observed by traditional optical microscopes. The reason is that limited by the diffraction limit, the volume of the focused spot cannot reach the required size. Among those seeking to improve this situation, the specificity of fluorescent probes makes two-photon fluorescence technology an important technology in the fields of life science, semiconductor technology, optical storage and flat-panel microfabrication. However, due to the dependence of secondary excitation on the intensity of light i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B27/58G02B27/28
Inventor 匡翠方库玉龙刘旭章海军张冬仙李海峰
Owner ZHEJIANG UNIV
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