Light emitting diode induced fluorescence detection device
A light-emitting diode, induced fluorescence technology, applied in the field of analysis and detection, can solve the problems of limiting laser-induced fluorescence technology, difficult to find fluorescent dyes, large volume, etc., and achieve the effect of avoiding the influence of external light sources
Active Publication Date: 2013-04-10
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology
Among them, the laser-induced fluorescence technology has extremely high sensitivity, but the disadvantages of high cost, large volume, and high energy consumption of the laser limit the application of the laser-induced fluorescence technology to a large extent.
Semiconductor lasers have volume and cost advantages, but currently their emission wavelengths are mainly limited to the long wavelength region, and it is difficult to find suitable fluorescent dyes in this region
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[0016] The present invention will be described in detail below with reference to the accompanying drawings and examples.
[0017] The invention provides a light-emitting diode-induced fluorescence detection device, which includes a gland 1 , a self-calibration platform 2 , a fluorescence collection lens 3 , a photodetector connector 4 , a filter 5 , a photodetector 6 and a bracket 7 . The upper end of the self-calibration platform 2 has an external thread and two U-shaped grooves, and two threaded holes are opened on its central axis, and the diameter of the lower through hole is larger than that of the upper end; the fluorescence collecting lens 3 has an external thread; the photodetector connector 4. There are external threads, the upper end is provided with a counterbore, and the lower end is provided with a mounting groove.
[0018] The inner thread of the gland 1 is mated with the outer thread on the upper end of the self-calibration platform 2; the threaded hole at the u...
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The invention relates to a light emitting diode induced fluorescence detection device and belongs to the technical field of analysis and detection. The light emitting diode induced fluorescence detection device comprises a gland, a self-calibrating platform, a fluorescence collection lens, a photoelectric detector connection piece, a light filter, a photoelectric detector and a bracket, wherein the gland is connected with the self-calibrating platform through a screw thread; a through hole at the upper end of the self-calibrating platform is connected with the fluorescence collection lens through a screw thread; U-shaped slots at the upper end of the self-calibrating platform are used for installing a capillary tube and an optical fiber respectively; a through hole at the lower end of theself-calibrating platform is connected with the photoelectric detector connection piece through a screw thread; the light filter is arranged in a counter bore of the photoelectric detector connectionpiece; the photoelectric detector is arranged in an installation slot of the photoelectric detector connection piece through a screw; and the centers of the fluorescence collection lens, the light filter and the photoelectric detector are positioned on the same axial line. The light emitting diode induced fluorescence detection device overcomes the shortcomings of large volume and the like of theconventional laser induced fluorescence detector and can meet the requirements on high-precision center alignment and repeated positioning of the optical fiber and a detection channel and increase the number of excitation optical fibers as required so as to increase the detection limit of a system.
Description
technical field [0001] The invention relates to a fluorescent detection device induced by a light-emitting diode, which belongs to the technical field of analysis and detection and is mainly used for the analysis and detection of micro and trace substances in biology, chemistry, food and environment. Background technique [0002] Fluorescence detection is a commonly used high-sensitivity detection technology, which uses the characteristics of the analyte to produce fluorescence when excited by light of a specific wavelength for qualitative and quantitative detection. Among them, laser-induced fluorescence technology has extremely high sensitivity, but the disadvantages of high cost, large volume, and high energy consumption of lasers limit the application of laser-induced fluorescence technology to a large extent. Semiconductor lasers have volume and cost advantages, but currently their emission wavelengths are mainly limited to the long-wavelength region, and it is difficul...
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IPC IPC(8): G01N21/64
Inventor 李晓琼张志广邓玉林胡晓明
Owner BEIJING INSTITUTE OF TECHNOLOGYGY