Method for separating and extracting 2-keto-D-gluconic acid
A technology for gluconic acid and glucose, applied in the field of chemical separation, can solve the problems of low yield, large loss of raw materials, and large heat consumption, and achieve the effects of high crude product purity, improved total yield, and reduced energy consumption
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Embodiment 1
[0030] 1. Flow the fermented broth (pH 1.37) with the content of 25.00 g / 100mL of 2-keto-D-gluconic acid removed from inorganic acid ions through the regenerated 150 mL D301 weakly basic anion at a flow rate of 1.0 BV / h The exchange resin bed, when the content of 2-keto-D-gluconic acid in the effluent was 24.10 g / 100mL, the adsorption was stopped, and 460 mL of fermentation broth (containing 115.0 g of 2-keto-D-gluconic acid) was collected. The effluent with pH﹤3.0 and an average content of 2-keto-D-gluconic acid of 17.85 g / 100mL totaled 290 mL (containing 51.77 g of 2-keto-D-gluconic acid);
[0031] 2. Use 290 mL of deionized water to push out the unadsorbed 2-keto-D-gluconic acid in the resin bed (converted to a net weight of 24.6 g containing 2-keto-D-gluconic acid);
[0032] 3. Use 95 mL of 40% methanol aqueous solution, 80 mL of 60% methanol aqueous solution and 240 mL of industrial methanol to flow through the resin bed at a flow rate of 3.0 BV / h to remove the water in t...
Embodiment 2
[0036] 1. Flow the fermented broth (pH 1.70) with the content of 10.00 g / 100mL of 2-keto-D-gluconic acid removed from inorganic acid ions through the regenerated 150 mL D315 weakly basic anion at a flow rate of 3.0 BV / h Exchange resin bed, when the content of 2-keto-D-gluconic acid in the effluent was 9.5 g / 100mL, the absorption and cross-exchange were stopped, and 1170 mL of fermentation broth (containing 117 g of 2-keto-D-gluconic acid) was collected. The effluent with pH﹤3.0 and the average content of 2-keto-D-gluconic acid was 7.73 g / 100mL totaled 810 mL (containing 62.61 g of 2-keto-D-gluconic acid).
[0037] 2. Use 240 mL of deionized water to push out the unadsorbed 2-keto-D-gluconic acid in the resin bed (converted to a net weight of 19.7 g containing 2-keto-D-gluconic acid);
[0038] 3. Use 75 mL of 54% methanol aqueous solution, 75 mL of 75% methanol aqueous solution and 210 mL of industrial methanol to flow through the resin bed at a flow rate of 3 BV / h to remove th...
Embodiment 3
[0042] 1. Flow the fermented broth (pH 1.54) with the content of 23.40 g / 100mL of 2-keto-D-gluconic acid removed from inorganic acid ions through the regenerated 150 mL D331 weakly basic anion at a flow rate of 1.2 BV / h The exchange resin bed, when the content of 2-keto-D-gluconic acid in the effluent was 22.61 g / 100mL, the adsorption was stopped, and 630 mL of fermentation broth (containing 147.42 g of 2-keto-D-gluconic acid) was collected. The effluent with pH﹤3.0 and an average content of 2-keto-D-gluconic acid of 17.62 g / 100mL totaled 472 mL (containing 83.17 g of 2-keto-D-gluconic acid);
[0043] 2. Use 240 mL of deionized water to push out the unadsorbed 2-keto-D-gluconic acid in the resin bed (converted to a net weight of 23.78 g containing 2-keto-D-gluconic acid);
[0044] 3. Use 80 mL of 51% methanol aqueous solution, 75 mL of 69% methanol aqueous solution and 220 mL of industrial methanol to flow through the resin bed at a flow rate of 3 BV / h to remove the water in t...
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