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Preparation method for rhesus ovary and ovarian follicle generation model

A technology for generating models and rhesus monkeys, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, peptide/protein components, etc., to achieve definite and reproducible effects

Active Publication Date: 2015-07-15
WEST CHINA HOSPITAL SICHUAN UNIV +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the preparation of rhesus monkey ovary and follicle production models using LH

Method used

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  • Preparation method for rhesus ovary and ovarian follicle generation model
  • Preparation method for rhesus ovary and ovarian follicle generation model
  • Preparation method for rhesus ovary and ovarian follicle generation model

Examples

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experiment example 1

[0007] The preparation of experimental example 1 animal model of the present invention

[0008] Taking 4 female rhesus monkeys (A, B, F, H) at reproductive stage as the test subjects, standard FSH (Gonal F) (follicle-stimulating hormone α injection, FSH) as the reference, completely randomized design, in the same reproductive physiology Cycle, starting from the 2nd day of the menstrual cycle, A and H monkeys were injected subcutaneously with LH, 60IU / monkey / time, twice a day, for 7 consecutive days, and B and F monkeys were subcutaneously injected with Gonal F, 60IU / monkey / time , 2 times a day, for 7 consecutive days, on the 9th day of the menstrual cycle, the ovarian state of the test monkey was detected by B-ultrasound, and on the 10th day of the menstrual cycle, a one-time intramuscular injection of 1000 international units (IU) of human chorionic gonadotropin ( hCG), and 30 hours after the injection of hCG for egg collection, mature culture and in vitro fertilization. Thr...

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Abstract

The invention provides a preparation method for a rhesus ovary and ovarian follicle generation model. According to the method, female rhesus monkeys are used as test objects, and each rhesus monkey is injected with 60 IU of luteinizing hormone LH each time and is injected twice everyday for seven days successively to prepare the rhesus ovary and ovarian follicle generation model. The invention also provides application of luteinizing hormone LH in preparing medicines for activating ovary and stimulating ovarian follicle thereof to grow. According to the invention, monkeys are used in the method for preparing the model, background data in physiology, hematology, blood biochemistry and the like of monkeys are abundant and easily available, monkeys have a biological background resembling to human beings, and therefore, test results of monkeys can well indicate clinical reactions of medicines; multi-aspect and multi-angle assessment are carried out, and obtained results can well and objectively indicate drug action on a reproductive system; animals (monkeys) and instruments (a B-mode ultrasonoscope, a microscope, a cell culture device, etc.) used in the model are simple and easily available, and the model has versatility.

Description

technical field [0001] The invention provides a method for preparing a rhesus monkey ovary and follicle generation model. Background technique [0002] Luteinizing hormone LH is a glycoprotein hormone, and like FSH (follicle-stimulating hormone α injection, FSH), it is one of the main factors to induce ovulation. In female mammals, the main function of LH is to cooperate with FSH to promote the growth and maturation of follicles, participate in the synthesis of estrogen by endometrial cells, induce ovulation, promote luteinization, and increase blood flow (Zhang Dongjie, et al., Luteinizing Phytogenesis Preliminary study on the relationship between hormone gene (LH) and litter size traits, "Heilongjiang Animal Husbandry and Veterinary Medicine", 2005 Issue 5). Its role in ensuring ovarian steroid hormone synthesis and in triggering ovulation is well known. Zhang Yunshan, et al., The role of LH in follicle development and ovarian stimulation, Foreign Medical Family Planning...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/24A61P15/08
Inventor 王莉陈波刘斌王汉蓉岳焕勋李宏霞
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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