A kind of biocontrol bacteria strain for preventing and treating poplar canker and its application

A technology of poplar canker and biocontrol bacteria, applied in the direction of application, biocide, microorganism, etc., can solve the problems of destruction, environmental pollution, etc., and achieve the effects of simple cultivation conditions, easy industrial production, and good development and application prospects

Active Publication Date: 2017-12-26
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pollution and destruction of the environment by chemical control has become a problem that cannot be ignored.

Method used

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  • A kind of biocontrol bacteria strain for preventing and treating poplar canker and its application
  • A kind of biocontrol bacteria strain for preventing and treating poplar canker and its application
  • A kind of biocontrol bacteria strain for preventing and treating poplar canker and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0027] Example 2 Trichoderma chrysogreen CGMCC8891 strain fermentation process optimization and stability

[0028] After single factor experiment, orthogonal design and response surface analysis, the optimal fermentation medium and conditions of CGMCC8891 were finally determined as follows: potato juice 200g / 1000mL, fructose 1%, beef powder 1.4%, KCl 0.1%, starting The pH value is 7.0, the temperature is 34.14°C, the rotation speed is 188.80r / min, the inoculum size is 3.58%, and the liquid volume is 100mL / 250mL. The predicted optimal antibacterial rate is 85.5667%. In order to facilitate operation and control in actual production, the fermentation conditions of CGMCC8891 were adjusted to an initial pH value of 7.0, a temperature of 34°C, a rotational speed of 190r / min, an inoculum size of 3.5%, and a liquid volume of 100mL / 250mL. The antibacterial rate of the optimized strain CGMCC8891 can reach 84.98%, which improves the antibacterial activity of the biocontrol strain and pro...

Embodiment 3

[0030] The preparation of the former powder of embodiment 3 trichoderma chrysanthemum CGMCC8891 biocontrol agent

[0031] The raw powder of bacterial agent was prepared by solid fermentation. 3 different solid fermentation matrix formulas are set: I. solid material 400g, wherein broken rice 50%, wheat bran 50%, and the ratio of solid material and water is 1: 1 (W / V), II. solid material 400g, wherein Broken rice 50%, corn flour 50%, and the ratio of solid material to water is 1:1 (W / V), III. 400g of solid material, wherein wheat bran 50%, cornmeal 50%, and the ratio of solid material to water It is 1:1 (W / V). The results showed that 50% corn flour mixed with 50% wheat bran was the most suitable solid fermentation substrate, the antibacterial rate was 88.24%, and the number of live spores reached 1.51×10 10 cfu / g.

[0032] The screening of solid fermentation conditions adopts an orthogonal design with three factors and three levels, and a total of 9 groups of experiments are ...

Embodiment 4

[0034] The development of embodiment 4 Trichoderma chrysogreen CGMCC8891 biocontrol agent

[0035] Adding 38wt% raw powder, 38wt% kaolin, 13wt% sodium lignosulfonate, 8.5wt% SDS, and 2.5wt% humic acid was the optimal formulation of the bacterial agent. The wettability, dispersibility, and suspension of the bacterial agent under this formula can reach the best level, and the stability to ultraviolet rays is also the strongest. The number of viable bacteria in the finished bacterial agent is 6.25×10 10 cfu / g, the antibacterial rate reached 89.12%. It has been determined that the shelf life of the bacterial agent is longer, and the bacteriostatic rate can still reach 75.38% after 6 months of storage. The interforest use method and effect of embodiment 5 Trichoderma chrysogreen CGMCC8891 biocontrol agent

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Abstract

The invention discloses a biocontrol bacterial strain for preventing and treating poplar canker and an application method thereof. The strain is Trichoderma aureoviride, and the strain number is CGMCC8891. The fermented liquid of this strain is mixed with wheat bran and corn flour, and after 7 days of fermentation, it is naturally air-dried and ground to make raw powder; add 38wt% raw powder, 38wt% kaolin, 13wt% sodium lignosulfonate, 8.5wt% SDS, 2.5wt% % humic acid to make bacterial agent; the diluted bacterial agent can be used to prevent and treat poplar canker in the way of spraying and root irrigation. The strain has the advantages of various modes of action on pathogenic bacteria, fast propagation speed, and stable effect. It has a strong inhibitory effect on the pathogenic bacteria of poplar canker, Botryosphaeria dothidia, and is of great significance to the prevention and treatment of poplar canker. , has good development and application prospects.

Description

Technical field: [0001] The invention relates to the field of plant protection, in particular to a biocontrol fungus strain for preventing and treating poplar canker and its application, in particular to a biocontrol strain of Trichoderma chrysogreen CGMCC8891 and its application for efficiently preventing and treating poplar canker. technical background: [0002] As an important processing raw material for wood-based panels and fiber materials, various varieties of poplar and their hybrids are widely distributed in the world. In addition, poplar plays an important role in afforestation, ecological restoration, landscape greening and windbreak and sand fixation. Poplar is susceptible to various diseases, among which poplar canker is one of the major biological disasters in poplar plantations in my country, which seriously restricts the development of poplar plantations. This disease is mainly caused by fungi such as Botryosphaeria spp. Poplar canker can cause the death of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/145C12R2001/885
Inventor 杨蕾梁军倪杨张星耀
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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