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Method for preparing small forensic insect specimens

A technology for insects and specimens, applied in the field of small forensic insect specimens, can solve the problems of discoloration of specimens, difficulty in accurately identifying the development time of pupae, decolorization, etc., and achieve the effect of easy transportation and storage

Inactive Publication Date: 2012-03-28
SHENYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two preservation solutions will cause discoloration or decolorization of the specimen, especially the effect on the color of the compound eyes of the nude pupa is more obvious
75% ethanol will quickly make the yellow compound eyes of naked pupae become colorless, while 10% formalin can fix the yellow compound eyes, but it can cause the red compound eyes to change into yellow compound eyes, making it difficult to accurately identify the development time of pupae , and even lead to wrong inference of the time of death

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preserve adult specimens as follows:

[0020] (1) Fix live insects with 6% glutaraldehyde for 60 minutes;

[0021] (2) Rinse the fixed insects with distilled water for 5 minutes;

[0022] (3) The washed insects were transferred to the embedding agent (2% sodium alginate, 0.45% NaCl, 0.008% CaCl 2 , 0.009% KCl, 0.013% MgSO 4， Penicillin 100IU / ml);

[0023] (4) Use a pipette or dropper to drop the drop containing the insect specimen into 2% CaCl 2 medium, shake gently for 30 minutes;

[0024] (5) Rinse in distilled water for 15 minutes to form transparent rigid balls containing insects.

Embodiment 2

[0026] Preserve specimens that are pupae as follows:

[0027] (1) Fix live insects with 6% glutaraldehyde for 60 minutes;

[0028] (2) Rinse the fixed insects with distilled water for 5 minutes;

[0029] (3) The washed insects were transferred to the embedding agent (1.5% sodium alginate, 0.65% NaCl, 0.012% CaCl 2 , 0.014% KCl, 0.02% MgSO 4， Penicillin 50IU / ml);

[0030] (4) Use a pipette or dropper to drop the liquid containing the insect specimen into 2.5% CaCl 2 medium, shake gently for 20 minutes;

[0031] (5) Rinse in distilled water for 20 minutes to form transparent rigid balls containing insects.

Embodiment 3

[0033] Take out the embedded pupae, do further dissection and then save them according to the following steps:

[0034] (1) Transfer the pellet containing the specimen into 20% sodium citrate solution and shake gently for 5 minutes to release the specimen;

[0035] (2) Dissect out naked pupae in Ren's solution;

[0036] (3) Rinse with distilled water for 5 minutes;

[0037] (4) The washed pupae were transferred to the embedding agent. The rinsed insects were transferred to the embedding agent (2% sodium alginate, 0.65% NaCl, 0.012% CaCl 2 , 0.014% KCl, 0.02% MgSO 4， Penicillin 100IU / ml);

[0038] (5) Use a pipette or dropper to drop the drop containing the insect specimen into 2.5% CaCl 2 medium, shake gently for 20 minutes;

[0039] (6) Rinse in distilled water for 15 minutes to form transparent rigid balls containing insects.

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Abstract

The invention relates to a method for preparing small insect specimens, and in particular relates to a method for preparing small forensic insect specimens. The method comprises the following steps: fixing live insects with fixing solution for 45-60 minutes; rinsing the fixed insects with distilled water for 5 minutes; transferring the rinsed insects to an embedding agent; dropping drops containing the insect specimens into 2-2.5% of CaCl2 by utilizing a pipettor or a dropper and gently shaking the vessel for 20-30 minutes; and transferring the products to the distilled water to be rinsed for 15-20 minutes, thus forming transparent rigid spheres containing insects. The method has the following advantages and effects: the original colors and morphological structures of the insects can be maintained; the specimens can not be decayed and are convenient to transport and store; and the embedded specimens can be taken out by a depolymerizing agent to be further studied.

Description

technical field [0001] The invention relates to a method for preparing an insect specimen, in particular to a method for preparing a small forensic insect specimen. Background technique [0002] Accurate inference of the time of death has always been a difficult problem in forensic science. It has been considered an effective method to infer the death time of the deceased by using the growth and development of insects on the corpse. At present, the preservation method of forensic insect specimens is immersion preservation, and the preservation solution is mainly 75% ethanol and 10% formalin. However, these two preservation solutions will cause discoloration or decolorization of the specimen, especially the effect on the color of the compound eyes of the nude pupa is more obvious. 75% ethanol will quickly make the yellow compound eyes of naked pupae become colorless, while 10% formalin can fix the yellow compound eyes, but it can cause the red compound eyes to change into y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 冯典兴刘广纯
Owner SHENYANG UNIV
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