Migratory insect yelk protein detection test paper based on colloidal gold chromatography and preparation method thereof

Abstract

The invention provides a migratory insect yelk collagen protein detection test paper based on colloidal gold chromatography and a preparation method thereof. The test paper is characterized in that a nitrocellulose coating film overlapped with a gold-labelled combined pad, a sample pad and an absorbent pad is arranged on a PVC (polyvinyl chloride) plastic bottom plate; The nitrocellulose coating film is provided with a detection line coated with a polyclonal antibody and a quality control line coated with a goat anti mouse IgG; and the gold-labelled combined pad is coated with a colloidal gold grain labelled antibody. The preparation method comprises the following steps: firstly preparing a monoclonal antibody, the polyclonal antibody and the goat anti mouse IgG and utilizing the monoclonal antibody to prepare the colloidal gold grain labelled antibody; then sequentially overlapping and pasting the sample pad, the gold-labelled pad, the nitrocellulose coating film and the absorbent pad on the PVC plastic bottom plate, wherein the nitrocellulose coating film is provided with the detection line and the quality control line; and coating the detection line with the polyclonal antibody, coating the quality control line with the goat anti mouse IgG and coating the gold-labelled combined pad with the colloidal gold grain labelled antibody, thus obtaining the detection test paper. The test paper provided by the invention is convenient and efficient and can be used for semi-quantitatively detecting the content of the yelk collagen protein in a migratory insect sample within 5-10 minutes so as to determine the source property of the migratory insect.

Description

technical field [0001] The invention relates to a test paper for detecting vitellogenin (Vg) of migratory insects by colloidal gold chromatography and a preparation method thereof. Background technique [0002] Rice leaf roller (Cnaphalocrocis medinalis Guenée) is an important migratory pest widely distributed in Asia and East Africa. It is distributed in almost all rice producing areas in my country, but there are obvious regional differences in the degree of occurrence. The hazard area is south of the line from Taiyi Mountain to Qinling Mountains. Adult rice leaf rollers have long-distance migratory habits, and there are 5 northward migrations and 3 southward migrations in my country every year. The large-scale migration makes the harm of the rice leaf roller sudden, and the prediction is extremely difficult, and it is often caught off guard and causes heavy losses. Therefore, it is very important to predict it accurately, timely and accurately. [0003] Forecasting and f...

AI Technical Summary

Problems solved by technology

However, there are defects such as heavy workload, low accuracy, obvious human factors, and the need for professional equipment in the actual operation of dissecting the ovary, which hinders the accuracy of the rice leaf roller. It is necessary to develop a convenient, fast and specific method. Forecasting method

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