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Application of protocatechualdehyde to preparation of medicaments for treating human neurodegenerative diseases

A technology for neurodegenerative and protocatechuic aldehydes, applied in nervous system diseases, drug combinations, active ingredients of aldehydes, etc., can solve the problems of anti-oxidative stress protection and Parkinson's disease application prospects without literature reports

Inactive Publication Date: 2012-05-16
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there are no literature reports on the protective effect of protocatechuic aldehyde against oxidative stress in nerve cells, its mechanism, and its application prospects in the prevention and treatment of Parkinson's disease

Method used

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  • Application of protocatechualdehyde to preparation of medicaments for treating human neurodegenerative diseases
  • Application of protocatechualdehyde to preparation of medicaments for treating human neurodegenerative diseases
  • Application of protocatechualdehyde to preparation of medicaments for treating human neurodegenerative diseases

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experiment example

[0024] 1. Experimental materials and experimental methods

[0025] 1.1. Cell culture

[0026] Human neuroblastoma cells SH-SY5Y (purchased from the ATCC cell bank in the United States) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) medium containing 10% bovine serum, and the culture conditions were 37°C, 5% CO 2 incubator.

[0027] 1.2. Analysis of cell viability by MTT method

[0028] SH-SY5Y cells contain 1x10 in 100ulDMEM per well 4 The density of cells was seeded in a 96-well plate, and after 24 hours, after the cells were attached to the bottom of the plate, different concentrations of protocatechualdehyde (5uM, 10uM, 20uM) were added and incubated in an incubator for 24 hours. Then add 10ul of different concentrations of H 2 o 2 (325uM, 350uM, 375uM) for 3 hours to induce oxidative stress. Afterwards, 10 ul Cell Counting Kit-8 (CCK-8) reagent (purchased from DOJINDO, Japan) was added to incubate for 4 hours, and the absorbance value was detected at 450 ...

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Abstract

The invention discloses application of protocatechualdehyde to preparation of medicaments for treating human neurodegenerative diseases. The invention first discovers that the protocatechualdehyde has activity of interacting with parkinsonism-related protein DJ-1 and further discovers that the protocatechualdehyde has the effects of resisting oxidative stress, preventing apoptosis of SH-SY5Y neurocyte, induced by the oxidative stress, restricting active oxygen in the neurocyte from generating, and the like through experiments. Proved by experimental results, the effect of the protocatechualdehyde to the neurocyte is mediated by the DJ-1 protein, and the protocatechualdehyde has an exact neuroprotective effect and can be used for preventing or treating the neurodegenerative diseases comprising parkinsonism.

Description

technical field [0001] The present invention relates to the use of protocatechualdehyde in the preparation of human neuroprotective drugs, in particular to the use of protocatechualdehyde in drugs for the prevention or treatment of human neurodegenerative diseases induced by oxidative stress damage of nerve cells, which belongs to the original Fields of pharmacological use of catechins. Background technique [0002] Parkinson's disease (PD) is a common neurodegenerative disease, and its main pathological features are the degeneration and loss of dopamine neurons in the substantia nigra pars compacta and the formation of Lewy bodies. The clinical symptoms of Parkinson's disease are mainly motor symptoms such as resting tremor, bradykinesia, muscle rigidity, and abnormal posture and gait. In addition, it can also be accompanied by mental symptoms such as depression and personality changes. The etiology and pathogenesis of PD are very complex, and have not been thoroughly stud...

Claims

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Application Information

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IPC IPC(8): A61K31/11A61P25/00A61P25/16
Inventor 蒲小平有贺宽芳高建伟
Owner PEKING UNIV
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