31 results about "SH-SY5Y" patented technology

The invention relates to cell transdifferentiation technology in the field of cell research, in particular to a method for inducing neuroblastoma cells to differentiate into neurons. Neuroblastoma cells were cultured with induction medium for 3-7 days to obtain neuronal cells; induction medium was obtained by adding retinoic acid to 10 μM to neural stem cell conditioned medium; DMEM / F12 basal medium, B27 , bFGF, EGF, heparin sodium, L-glutamine, and penicillin-streptomycin complete medium for culturing neural stem cells, cultured for 3 days and half of the complete medium was replaced, and the replaced culture medium was the neural stem cell conditioned medium. The method of the invention improves the differentiation efficiency, improves the maturity of the cells and the synapse growth of the neuron cells, and can inhibit the apoptosis in the SH-SY5Y cells treated with RA.

The invention discloses a novel application of red stilbene and red stilbene polysaccharide in preparing medicament for treatment of Alzheimer disease. Red stibene and red stilbene polysaccharide in the invention can protect SH-SY5Y cells from the damage from A beta 25-35, and increase the viability of SH-SY5Y cells. The Morris water maze test indicates that red stibene and red stilbene polysaccharide can improve the cognitive dysfunction of mice with rapid brain aging and rats injected with A beta and AD in the hippocampus. Further histology detection indicates that red stilbene and red stilbene polysaccharide can increase the contents of center neurotransmitters 5-HT, 5-HIAA, NE and DA in rapid aging mice brain, wherein the neurotransmitters are monoamines.

The invention provides an application of a bitter orange volatile oil extract in preparation of an antidepressant drug. The bitter orange volatile oil extract is extracted from a bitter orange medicinal material, and a main component is limonene. An extraction technology of the bitter orange volatile oil extract comprises steps as follows: the bitter orange medicinal material is taken and soaked for 1-3 h, a steam distillation method is adopted, 6-10 times of water in volume is added for extraction for 5-9 h, and the bitter orange volatile oil extract is obtained. A preferable technology comprises steps as follows: the bitter orange medicinal material is taken and soaked for 3 h, the steam distillation method is adopted, 8 times of water in volume is added for extraction for 7 h, and the bitter orange volatile oil extract is obtained. Multiple preparations can be prepared from the bitter orange volatile oil extract, and the bitter orange volatile oil extract can be applied to development of a clinical new antidepressant drug after addition of pharmaceutically acceptable auxiliary materials and carriers. According to the application of the bitter orange volatile oil extract in preparation of the antidepressant drug, in-vitro pharmacological experiments prove that the bitter orange volatile oil extract has a protection effect on hypoxia-injury SH-SY5Y nerve cells, has a remarkable anti-depression function and lays the foundation for development of the new antidepressant drug.

The invention belongs to the technical field of biomedicine, and relates to a novel target used for preventing enterovirus 71 infection, and applications thereof. According to the invention, SH-SY5Y is taken as the target cell, and RNA interference technology is adopted for down-regulation of expression of target cell host protein so as to search host factors capable of inhibiting EV71 infection of SH-SY5Y cells effectively and protect the functions of central nervous systems. It is fond by experiments that calcium-and inte-grin-binding protein, CIB1 molecular possesses significant importance in EV71 infection of SH-SY5Y, and down-regulation of expression of CIB1 is capable of inhibiting EV71 infection obviously. The invention also provides applications of CIB1 in preparing drugs used for preventing or treating EV71 infection.

The invention relates to an extraction method of an antioxidant and an application in protection of nerve cells against oxidation by utilizing the antioxidant, belonging to the technical field of biological medicines. The extraction method disclosed by the invention comprises the following steps of: taking myricaria laxiflora as a raw material, taking ethanol as an extraction solvent, performing Soxhlet extraction, performing cold leaching on a crude extraction solution with HCl, adjusting the pH with saturated NaCO3, extracting with chloroform, performing chloroform-methanol gradient elution by silica gel column chromatography, performing Sephadex LH-20 gel column methanol elution, and further performing crystallization and re-crystallization to obtain the antioxidant. The antioxidant is identified as 3, 4-dimethoxy-5-methylparaben by 1H-NMR and 13C-NMR, the molecular formula is C10H12O5, and the anti-oxidation property and the free radical scavenging activity are stronger. The concentration of the antioxidant is 0.5-12.5mu g / mL, when the dosage is 100mu L, the toxicity of the natural antioxidant against SH-SY5Y cells is less than 3.4%, the relative survival rate against human neuroblastoma cells SH-SY5Y after oxidative damages by hydrogen peroxide is 50-80%, and the antioxidant has a better anti-oxidation protection effect in comparison with PG (propyl gallate), can be used as a natural non-toxic antioxidant and can be applied to the fields of foods, medicines, cosmetics and the like.

The invention relates to an extraction method for (22trans)-3beta-hydroxy-cholest-5,22-diene-24-one in Caulerpa racemosa. According to the present invention, the compound is obtained from Caulerpa racemosa by adopting extracting, pressure reducing concentrating, extracting, and silica gel column chromatography and gel column chromatography purification, the structure of the compound is determined through physicochemical constant determination and spectrum data analysis, and results of a plurality of in vitro nerve protection effect experiments of SH-SY5Y cell damage prove that the compound provides the significant protection effect on the beta-amyloid 25-35(A[beta]25-35) damaged SH-SY5Y cells, and can be used for preparation of nerve protection agents and Alzheimer's disease preventing drugs.