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Application of dynamin 1 in prevention and treatment on enterovirus type 71 infection

A dynamin, enterovirus technology for biomedical applications

Active Publication Date: 2016-04-20
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] At present, there is no research report on the role of DNM1 molecules in EV71 infection of human neuroblastoma (SH-SY5Y). In-depth research on DNM1 molecules can not only improve the understanding of EV71 infection and pathogenic mechanisms, but also contribute to prevention and treatment. Treatment of EV71 infection provides new ideas and targets

Method used

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  • Application of dynamin 1 in prevention and treatment on enterovirus type 71 infection
  • Application of dynamin 1 in prevention and treatment on enterovirus type 71 infection
  • Application of dynamin 1 in prevention and treatment on enterovirus type 71 infection

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Experimental program
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Embodiment 1

[0048] 1 Design and synthesis of specific siRNA sequences for each host cell molecule.

[0049] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis of each target gene, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each gene, a...

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Abstract

The invention relates to the technical field of biomedicine, and provides a new target for resisting entervirus type 71 infection and application. A human neuroblastoma cell (SH-SY5Y) is taken as a target cell, expression of host protein of the target cell is down-regulated by adopting an RNA interference technique to look for a host factor which can effectively inhibit the EV71-infected human neuroblastoma cell (SH-SY5Y), and therefore the function of a central nervous system is protected. Through experiments, it is found that a dynamin 1 (NDM1) plays an important role on the EV71-infected SH-SY5Y, and EV71 infection can be obviously inhibited by down-regulating expression of the DNM1. The invention provides application of the DNM1 in preparation of medicine for preventing or treating the entervirus type 71 infection.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new target and application of anti-enterovirus 71 infection. Background technique [0002] Enterovirus 71 (enterovirus71, EV71) belongs to the Picornaviridae Enterovirus genus Human Enterovirus A, and is one of the main pathogens that cause Hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand, foot and mouth disease in several major provinces and cities in 2008, the number of people infected and the death rate of the disease have remained high, with more than 1 million cases and nearly 1,000 deaths reported every year. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms in the hands, feet, buttocks and oral mucosa; a small numbe...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/7105A61P31/14
CPCA61K31/7105
Inventor 彭浩然朱勇喆朱耐伟徐庆强戚中田宋洪元
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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