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Extraction method of (22trans)-3β-hydroxy-cholesta-5,22-dien-24-one from Pteris racemosus

A technology of Pteris racemosa and hydroxyl, which is applied in the field of extracting -3β-hydroxy-cholesta-5,22-dien-24-one and its preparation, can solve problems that have not yet been seen, and achieve the prevention of Alzheimer's disease disease effect

Active Publication Date: 2016-06-22
南通药享科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And, so far, there is no research report on the biological activity of the compound (22trans)-3β-hydroxyl-cholesta-5,22-dien-24-one

Method used

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  • Extraction method of (22trans)-3β-hydroxy-cholesta-5,22-dien-24-one from Pteris racemosus
  • Extraction method of (22trans)-3β-hydroxy-cholesta-5,22-dien-24-one from Pteris racemosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 (22 trans)-3β - Preparation of Hydroxy-cholesta-5,22-dien-24-one

[0022] (1) the frozen Chinese fern raceme ( C. racemosa ) (collected from the coast of Zhanjiang, Guangdong) 5kg (dry weight) was thawed at room temperature, extracted three times by percolation with 30L95% ethanol, each percolation for 24 hours, and combined the extracts;

[0023] (2) Concentrate the above extract under reduced pressure at a temperature ≤ 45°C to recover ethanol to obtain 350 g of crude extract; disperse the crude extract in water to form a suspension, and use petroleum ether (1.5 L), Ethyl acetate (1.5L) and n-butanol (1L) were extracted three times respectively, and the obtained extracts were concentrated under reduced pressure to obtain petroleum ether extract (38g), ethyl acetate extract (160g) and n-butanol extract ( 120g);

[0024](3) Carry out silica gel column chromatography with ethyl acetate extract, with sherwood oil / acetone gradient elution, the separated compo...

Embodiment 2

[0025] Example 2 (22 trans)-3β-hydroxyl-cholesta-5,22-diene-24-one on Aβ 25-35 Induced protective effect of SH-SY5Y cell injury

[0026] 1. Experimental samples and experimental methods

[0027] Preparation of test sample solution: The test sample is the pure compound (22trans)-3β-hydroxy-cholesta-5,22-dien-24-one prepared in Example 1 above. Accurately weigh an appropriate amount of sample and prepare a solution with the desired concentration with DMSO for pharmacological activity testing.

[0028] Cell line and subculture of cells: The SH-SY5Y cell line (purchased from ATCC (American type culture collection) in the United States) was used for the activity test. Subculture in DMEM medium containing 10% FBS in an incubator with 5% carbon dioxide at 37°C.

[0029] Cell activity test method (MTT method): the present invention adopts MTT method, and the test evaluates the tested sample to Aβ 25-35 Protective effect leading to decreased viability of SH-SY5Y cells. Dehydrogena...

Embodiment 3

[0037] Get the compound obtained in Example 1, add commonly used pharmaceutical excipients, and make dosage forms such as tablets, capsules, and oral preparations.

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Abstract

The invention relates to an extraction method for (22trans)-3beta-hydroxy-cholest-5,22-diene-24-one in Caulerpa racemosa. According to the present invention, the compound is obtained from Caulerpa racemosa by adopting extracting, pressure reducing concentrating, extracting, and silica gel column chromatography and gel column chromatography purification, the structure of the compound is determined through physicochemical constant determination and spectrum data analysis, and results of a plurality of in vitro nerve protection effect experiments of SH-SY5Y cell damage prove that the compound provides the significant protection effect on the beta-amyloid 25-35(A[beta]25-35) damaged SH-SY5Y cells, and can be used for preparation of nerve protection agents and Alzheimer's disease preventing drugs.

Description

technical field [0001] The present invention relates to a kind of extracting (22trans)-3β-hydroxyl-cholesta-5,22-dien-24-one from Racemosa and its preparation method and use; Aβ 25-35 The damaged SH-SY5Y cells have protective active ingredients, so they can be used to prepare neuroprotective drugs and drugs for preventing Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a primary degenerative disease of the central nervous system, which affects more than 35 million people worldwide. Its clinical manifestations are progressive memory impairment, motor impairment, cognitive impairment, etc. (Prince, M.eral, World Alzheimer Report 2013 ; Alzheimer's Disease International: London, UK, 2013; pp1?92). There are about 8 million AD patients in my country. Because advanced AD patients cannot take care of themselves and are often accompanied by severe mental and neurological symptoms, it not only seriously reduces the quality of life of the elderly, b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00A61K31/575A61P25/28A61P25/00
CPCC07J9/00
Inventor 毛水春章海燕郭跃伟刘定权
Owner 南通药享科技有限公司
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