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Application of 5,7-dihydroxy-4'-methoxy flavone preparation extracted from snow lotus in preparation of medicament for treating ischemic stroke

A technology of methoxyflavone and snow lotus, applied in the preparation of drugs for the treatment of ischemic stroke, in the field of preparation of functional medicines extracted from snow lotus, can solve the problem of less Acacetin

Inactive Publication Date: 2013-04-24
朱沂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on Acacetin, mainly focusing on the research of anti-tumor effect

Method used

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  • Application of 5,7-dihydroxy-4'-methoxy flavone preparation extracted from snow lotus in preparation of medicament for treating ischemic stroke
  • Application of 5,7-dihydroxy-4'-methoxy flavone preparation extracted from snow lotus in preparation of medicament for treating ischemic stroke
  • Application of 5,7-dihydroxy-4'-methoxy flavone preparation extracted from snow lotus in preparation of medicament for treating ischemic stroke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of 5,7-dihydroxy-4'-methoxyflavone preparation extracted from Snow Lotus

[0032] Take the pulverized snow lotus, add 80% ethanol whose volume is 15-20 times of the weight of snow lotus, put it into a microwave extraction device and carry out microwave extraction for 2-3 times, recover and concentrate the ethanol under reduced pressure, add it to a macroporous adsorption resin column for adsorption, 60% ethanol was eluted, and 5 times the volume of the eluate was collected, the ethanol was recovered under reduced pressure and concentrated, added to a polyamide chromatography column, eluted with a mixed solvent of dichloromethane-methanol with a volume ratio of 4:1, and washed with the same The polyamide film is developed with the above-mentioned dichloromethane-methanol eluent as a supporting test, collects the fraction of Rf=4-5 on the polyamide film, recovers the solvent under reduced pressure, adds acetone to crystallize, separates the crystal, ...

Embodiment 2

[0033] Example two: Extract 5,7-dihydroxy-4'-methoxyflavone preparation from Snow Lotus

[0034] Take 1 kg of snow lotus, crush it, add 15 times its mass and volume of ethanol solution with a concentration of 80%, put it into a microwave extraction device and carry out microwave extraction for 3 times, with an extraction power of 300w, each time for 10 minutes, combine the extracts, and recover ethanol under reduced pressure And concentrated, added to the macroporous adsorption resin column for adsorption, 60% ethanol gradient elution, collected 5 times the column volume eluate, recovered ethanol under reduced pressure and concentrated, added to the polyamide chromatography column, with a ratio of 4 : 1 dichloromethane-methanol mixed solvent elution, use the same kind of polyamide film to develop with the above-mentioned dichloromethane-methanol eluent as supporting detection, collect for the fraction of Rf=4-5 on the polyamide film, The solvent was recovered under reduced pre...

Embodiment 3

[0035] Example 3: Extracting 5,7-dihydroxy-4'-methoxyflavone preparation from Snow Lotus has neuroprotective effect in cerebral ischemia-reperfusion injury

[0036] 1. Experimental objects: C57 mice, male, weighing 20-25g, 8-12 weeks old, considering that estrogen has a neuroprotective effect on cerebral ischemia-ischemia-reperfusion injury. Therefore, in order to exclude the interference caused by estrogen, male mice were selected as the experimental subjects.

[0037] 2. Experimental grouping: randomly divided into 5 groups: sham operation group (n=18); ischemia / reperfusion model group (I / R group, n=18); Acacetin high dose group: 25mg / kg (n=18 ); Acacetin medium dose group: 12.5 mg / kg (n=18); Acacetin low dose group: 6.25 mg / kg (n=18). Acacetin was formulated with 1% DMSO and injected intraperitoneally once in mice after ischemia-reperfusion injury. The sham operation group was given an equal volume of medium dose of Acacetin at the same time point, and the ischemia / reperf...

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Abstract

The invention discloses an application of 5,7-dihydroxy-4'-methoxy flavone preparation extracted from snow lotus in preparation of a medicament for treating ischemic stroke. By effectively extracting the 5,7-dihydroxy-4'-methoxy flavone preparation from the snow lotus and establishing an ischemia reperfusion model of a mouse, the influence of acacetin on the infarct volume and the neurologic impairment caused by ischemia reperfusion injury of the mouse is observed. The study carried out by establishing an SH-SY5Y cell OGD model, testing the cell viability and the cell apoptosis, and demonstrating the integral level of the acacetin shows that the acacetin has the effect of significantly reducing the infarct volume, improving the neurologic function score, promoting the cell survival and reducing the cell apoptosis, clarifies that the acacetin has a neurologic protection effect on the cerebral ischemia reperfusion injury and proves that the 5,7-dihydroxy-4'-methoxy flavone preparation can be well applied to preparing the medicament for treating ischemic stroke and has wide application value.

Description

technical field [0001] The present invention relates to the technical field of preparation of functional medicines extracted from Saussurea indica, in particular to the application of a preparation of 5,7-dihydroxy-4'-methoxyflavone extracted from Saussurea indica in the preparation of medicines for treating ischemic stroke technology field. Background technique [0002] With its high morbidity, high mortality and high disability rate, stroke brings heavy burden and great suffering to patients, families and society. At present, it has become the fourth cause of death and the first cause of disability in the world. Every 6 seconds in the world, one person suffers from a stroke, and one person dies of a stroke every 4 minutes. The vast majority of stroke patients Disabled by it. Epidemiological data show that there are 1.5-2 million new stroke cases in my country every year. At present, there are more than 10 million stroke patients in my country, and about 70% of strokes a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40A61K31/352A61P9/10
Inventor 朱沂补娟胡刚
Owner 朱沂
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