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Polypeptide inhibitor for inhibiting aggregation and toxicity of beta amyloid protein and application of polypeptide inhibitor

An amyloid and toxicity technology, applied in the field of biomedicine, can solve the problems of no reports, and achieve the effect of reducing the content and inhibiting the fibrillar assembly behavior

Active Publication Date: 2015-01-14
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the direct interaction between ACTH and Aβ and the regulation of its aggregation process.

Method used

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  • Polypeptide inhibitor for inhibiting aggregation and toxicity of beta amyloid protein and application of polypeptide inhibitor
  • Polypeptide inhibitor for inhibiting aggregation and toxicity of beta amyloid protein and application of polypeptide inhibitor
  • Polypeptide inhibitor for inhibiting aggregation and toxicity of beta amyloid protein and application of polypeptide inhibitor

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Embodiment Construction

[0034] The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and through specific implementation methods.

[0035] Unless otherwise specified, the reagents used in the following examples are of analytical grade and can be purchased commercially from formal channels.

[0036] Unless otherwise specified, the neuroma cell line SH-SY5Y used in the following examples was purchased from the Basic Medical Cell Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.

[0037] Unless otherwise specified, the β-amyloid protein (Aβ) used in the following examples was purchased from Shanghai Gil Biochemical Technology Co., Ltd., and its purity was >95%.

[0038] Unless otherwise specified, the peptides used in the following examples were prepared by Shanghai Keyept Biotechnology Co., Ltd. using solid-phase peptide synthesis with a purity of >98%.

[0039] Unless otherwise specified, the ultr...

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Abstract

The invention discloses a polypeptide for inhibiting aggregation and toxicity of beta amyloid protein (A beta) or a variant of the polypeptide with polypeptide functions. The polypeptide has a strong binding force with the A beta protein, so that the effective content of a beta structure can be greatly reduced when A beta achieves an aggregation balanced state, the secondary structure of A beta in a solution environment can be changed, the content of the beta structure can be greatly reduced, even the beta structure disappears, the toxicity of A beta to SH-SY5Y cells can be remarkably reduced when the concentration of the polypeptide is extremely low, and generation of A beta-induced active oxygen can be greatly inhibited. Therefore, a feasible method is provided to treatment of Alzheimer disease caused by beta amyloid protein, and thought and reference standard can be provided to discovery of polypeptide pilot compounds for treatment of amyloid protein related diseases.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically relates to a polypeptide inhibitor for inhibiting aggregation and toxicity of beta amyloid protein and application thereof. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease, the main clinical manifestations are neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, personality changes and language barriers, which seriously affect social, occupation and life function, and even eventually lose the ability to live, which poses a huge threat to human health and life, causing a huge economic burden on families and society. Amyloid deposits (senile plaques) are found outside the nerve cells of AD patients, and β-amyloid protein (Aβ) is the main component of senile plaques. [0003] Aβ protein is composed of 39-43 amino acids with a molecular weight of about 4kDa. It is hydrolyzed from β-amyloid precursor pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/695C12N15/16A61K38/35A61P25/28G01N33/74G01N33/68
CPCA61K38/00C07K14/695
Inventor 张敏李平王晨轩杨延莲王琛
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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