Fat-soluble extract of ligularia fischeri and preparation method and application thereof

Abstract

The invention discloses a fat-soluble extract of ligularia fischeri and a preparation method and application thereof. The active monomer components of the extract comprise Bakkenolide A, ligularia hodgsonii aldehyde and luteolin, the fat-soluble extract of ligularia fischeri has the effects of inhibiting the proliferation of tumor cells SH-SY5Y and MCF-7, and also has a certain effect of scavenging DPPH free radicals, the application of medicines or health care products for resisting oxidation, delaying aging, improving organism immunity, preventing cancer and the like is provided, the fat-soluble extract of ligularia fischeri can also be used as natural antioxidants, and medicines for diseases such as obesity, hyperlipidemia, hypertension, diabetes, fatty liver, coronary heart disease, sleep apnea syndrome and the like caused by the obesity, and the application of the medicines and health care products related to the diseases are provided.

Description

technical field

[0001] The invention belongs to the technical field of medicinal plant extraction, and in particular relates to a fat-soluble extract of Ligularia sativa and its preparation method and application. Background technique

[0002] Ligularia fischeri (Ligularia fischeri) is a perennial herbaceous plant of the genus Ligularia in the family Compositae, also known as Ligularia fischeri and Ligularia fischeri. The plant is widely distributed in China and has been reported in Korea in recent years. The roots and rhizomes of this plant have many records of medicinal use, and they are called Aster asteroides. Its young leaves are also reported to be eaten in Northeast my country and South Korea. It has the functions of dispelling cold and moistening lung, resolving phlegm and relieving cough, regulating qi and promoting blood circulation, etc. It is used to treat lung abscess cough, bruises, joint abscess and other diseases. The main active ingredients in Ligularia lu...

Examples

Embodiment 1

[0020] Example 1 Preparation of Ligularia sativa fat-soluble extract

[0021] Take 50 g of the dried and crushed hoof leaf of Ligularia spp. collected from the mountainous area of ​​Guizhou, add 75% ethanol aqueous solution as the extraction solvent, and the ratio of solid to liquid is 1:10 (mass volume ratio, unit g / ml), and use the tissue crushing extraction method to extract, extract The time is 10s, filter, extract the dregs again in the same way, filter, combine the two filtrates, concentrate according to the ratio of 15:1, let stand for 12 hours, filter with suction, extract the filtrate with ethyl acetate and separate the ethyl acetate layer , remove the ethyl acetate solvent, and dry to obtain 1.5 g of a dark yellow solid, which is the fat-soluble extract of Ligularia sativa.

Embodiment 2

[0022] Example 2 Preparation of Ligularia sativa fat-soluble extract

[0023] Take 50 g of the dried and pulverized hoof leaf Lignus flower part collected from the mountainous area of ​​Guizhou, add 60% ethanol aqueous solution as the extraction solvent, the ratio of solid to liquid is 1:15 (mass volume ratio, unit g / ml), and extract by hot reflux extraction method. Time 1h, filter, extract the dregs again in the same way, filter, combine the two filtrates, concentrate according to the ratio of 10:1, stand for 6 hours, and filter with suction, the filtrate is mixed with silica gel through a 100-mesh column, and then mixed with petroleum ether, Ethyl acetate was extracted sequentially, the ethyl acetate solution was collected, the ethyl acetate solvent was removed, and dried to obtain 1.7 g of a dark yellow solid, which was the fat-soluble extract of Ligularia sativa.

Embodiment 3

[0024] The preparation of embodiment 3 monomer compound

[0025] Get 650g of the dried and pulverized Lignus chinensis flowers and flower stems collected from the mountainous area of ​​Guizhou (80-100g each time, divided into 7 times), add 75% ethanol aqueous solution as the extraction solvent, and the ratio of solid to liquid is 1:10 (mass volume ratio , unit g / ml), using the tissue crushing extraction method for extraction, the extraction time is 10s, filtering, the dregs are extracted once again by the same method, filtered, the two filtrates are combined, concentrated according to the ratio of 16:1, placed for 4 hours, and suction filtered , the filtrate was extracted with ethyl acetate, and the ethyl acetate layer was separated, and the ethyl acetate solvent was removed, and dried to obtain 23 g of a dark yellow solid, which was the fat-soluble extract of Ligularia sativa. Take 20g of the extract, go through silica gel column chromatography, use petroleum ether-ethyl acet...

technical field

[0001] The invention belongs to the technical field of medicinal plant extraction, and in particular relates to a fat-soluble extract of Ligularia sativa and its preparation method and application. Background technique

[0002] Ligularia fischeri (Ligularia fischeri) is a perennial herbaceous plant of the genus Ligularia in the family Compositae, also known as Ligularia fischeri and Ligularia fischeri. The plant is widely distributed in China and has been reported in Korea in recent years. The roots and rhizomes of this plant have many records of medicinal use, and they are called Aster asteroides. Its young leaves are also reported to be eaten in Northeast my country and South Korea. It has the functions of dispelling cold and moistening lung, resolving phlegm and relieving cough, regulating qi and promoting blood circulation, etc. It is used to treat lung abscess cough, bruises, joint abscess and other diseases. The main active ingredients in Ligularia lu...

Examples

Embodiment 1

[0020] Example 1 Preparation of Ligularia sativa fat-soluble extract

[0021] Take 50 g of the dried and crushed hoof leaf of Ligularia spp. collected from the mountainous area of ​​Guizhou, add 75% ethanol aqueous solution as the extraction solvent, and the ratio of solid to liquid is 1:10 (mass volume ratio, unit g / ml), and use the tissue crushing extraction method to extract, extract The time is 10s, filter, extract the dregs again in the same way, filter, combine the two filtrates, concentrate according to the ratio of 15:1, let stand for 12 hours, filter with suction, extract the filtrate with ethyl acetate and separate the ethyl acetate layer , remove the ethyl acetate solvent, and dry to obtain 1.5 g of a dark yellow solid, which is the fat-soluble extract of Ligularia sativa.

Embodiment 2

[0022] Example 2 Preparation of Ligularia sativa fat-soluble extract

[0023] Take 50 g of the dried and pulverized hoof leaf Lignus flower part collected from the mountainous area of ​​Guizhou, add 60% ethanol aqueous solution as the extraction solvent, the ratio of solid to liquid is 1:15 (mass volume ratio, unit g / ml), and extract by hot reflux extraction method. Time 1h, filter, extract the dregs again in the same way, filter, combine the two filtrates, concentrate according to the ratio of 10:1, stand for 6 hours, and filter with suction, the filtrate is mixed with silica gel through a 100-mesh column, and then mixed with petroleum ether, Ethyl acetate was extracted sequentially, the ethyl acetate solution was collected, the ethyl acetate solvent was removed, and dried to obtain 1.7 g of a dark yellow solid, which was the fat-soluble extract of Ligularia sativa.

Embodiment 3

[0024] The preparation of embodiment 3 monomer compound

[0025] Get 650g of the dried and pulverized Lignus chinensis flowers and flower stems collected from the mountainous area of ​​Guizhou (80-100g each time, divided into 7 times), add 75% ethanol aqueous solution as the extraction solvent, and the ratio of solid to liquid is 1:10 (mass volume ratio , unit g / ml), using the tissue crushing extraction method for extraction, the extraction time is 10s, filtering, the dregs are extracted once again by the same method, filtered, the two filtrates are combined, concentrated according to the ratio of 16:1, placed for 4 hours, and suction filtered , the filtrate was extracted with ethyl acetate, and the ethyl acetate layer was separated, and the ethyl acetate solvent was removed, and dried to obtain 23 g of a dark yellow solid, which was the fat-soluble extract of Ligularia sativa. Take 20g of the extract, go through silica gel column chromatography, use petroleum ether-ethyl acet...

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