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Application of baicalin

A technology of baicalin and medicine, which is applied in the application field of baicalin in the preparation of drugs for the prevention and treatment of Alzheimer's disease, and can solve problems such as the research report on the preparation of drugs for the prevention and treatment of Alzheimer's disease by baicalin

Inactive Publication Date: 2011-12-21
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant research report on the preparation of baicalin in the preparation of drugs for the prevention and treatment of Alzheimer's disease.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, the effect of baicalin on the secretion of Aβ protein in SH-SY5Y-APP695sw transgenic cells

[0035] Experimental materials: SH-SY5Y-APP695sw transgenic cells were preserved by the Natural Medicine Engineering Research Center of the Ministry of Education, Beijing Normal University; baicalin was purchased from China Institute for the Control of Pharmaceutical and Biological Products, and the HPLC detection content was >98%; G418 was purchased from Ameresco; cell culture reagents , Aβ40 Colorimetric ELISA Kit and Aβ42 Colorimetric ELISA Kit were purchased from Invitrogen.

[0036] Cell culture: SH-SY5Y cells were cultured in DMEM / F12 complete medium containing 10% fetal bovine serum, 100U / mL penicillin, and 100mg / L streptomycin in an incubator at 37°C, 5% CO2, and 90% relative humidity . An additional 500 μg / mL G418 was added to the medium to maintain the expression of exogenous genes.

[0037] Experimental method: SH-SY5Y-APP695sw cells were mixed with 2.5×10...

Embodiment 2

[0039] Embodiment 2, the influence of baicalin on APP protein processing and shearing

[0040] Experimental materials: SH-SY5Y-APP695sw and SH-SY5Y-C99 transgenic cells were preserved by the Natural Medicine Engineering Research Center of the Ministry of Education of Beijing Normal University; Ameresco Company; cell culture reagents were purchased from Invitrogen Company; BCA protein concentration assay kit was purchased from Saichi Biotechnology Company; anti-sAPPα monoclonal antibody 2B3, anti-sAPPβsw monoclonal antibody 6A1 and anti-APP polyclonal antibody were purchased from IBL Company; Total sAPP monoclonal antibody 22C11 was purchased from Millipore Company; anti-β-actin monoclonal antibody C4 was purchased from Santa Cruz Company; Amersham ECL plus chromogenic reagent was purchased from GE Company.

[0041] Cell culture: see Example 1.

[0042] Experimental method: SH-SY5Y-APP695sw and SH-SY5Y-C99 transgenic cells were divided into 2×10 5 Cells / well were seeded in 6-...

Embodiment 3

[0044] Embodiment 3, the effect of baicalin on the protein expression and enzyme activity of β-secretase (BACE1) in the cerebral cortex and hippocampus of dementia model mice

[0045] Experimental materials: APP695sw / PS1ΔE9 C57BL / 6J transgenic mice were purchased from the Institute of Experimental Animals, Chinese Academy of Medical Sciences, license number SCXK(Beijing) 2005-0013; Drink sterile drinking water at an ambient temperature of 20±2°C and a relative humidity of 45-70%; baicalin was purchased from China Institute for the Control of Pharmaceutical and Biological Products, and the HPLC detection content was >98%; anti-BACE1 polyclonal antibody was purchased from Merck; anti-β -actin monoclonal antibody C4 was purchased from Santa Cruz Company; Amersham ECL plus chromogenic reagent was purchased from GE Company; β-secretase enzyme activity assay kit was purchased from Biovision Company; the microplate reader was Thermo Labsystems Multiskan MK3.

[0046] Grouping and adm...

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Abstract

The invention relates to the field of traditional Chinese medicine, and discloses the application of baicalin in the preparation of drugs for preventing and treating Alzheimer's disease. Experiments have shown that baicalin can inhibit the secretion of Aβ protein and β-secretase in SH-SY5Y-APP695sw cells, the enzymatic activity of β-secretase and the enzymatic cleavage of β-secretase, thereby inhibiting the accumulation of Aβ protein. Simultaneous tests show that baicalin can improve the learning and memory ability, promote the degradation of oligomerized Aβ, thereby reducing the toxic effect of Aβ protein, and achieve the purpose of preventing and treating Alzheimer's disease. Therefore, the present invention provides baicalin in the preparation of prophylaxis and Application of drugs in the treatment of Alzheimer's disease.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to the application of baicalin in the preparation of drugs for preventing and treating Alzheimer's disease. Background technique [0002] Scutellaria baicalensis Georgi, also known as camellia root and Tujincha root, is a plant of the Labiatae family, and its roots are used as medicine. Studies have found that Scutellaria baicalensis contains a variety of flavonoids such as baicalin, baicalein, and wogonoside, which have antibacterial, antiviral, antipyretic, antihypertensive, diuretic, anti-inflammatory, and anti-allergic effects. . The clinical application of Scutellaria baicalensis is better than that of Coptis chinensis, and it does not produce drug resistance. [0003] Baicalin (Baicalin) is a flavonoid compound extracted and isolated from the root of Scutellaria baicalensis. It is the most abundant active ingredient in Scutellaria baicalensis. It belongs to glucur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P25/28
Inventor 张文生马涛
Owner BEIJING NORMAL UNIVERSITY
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