Protein chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment

A protein chip and interferon technology, applied in the field of protein chips, can solve the problems of high treatment cost and long medication cycle, and achieve the effects of convenient clinical application, convenient detection, and reduced pain for patients

Inactive Publication Date: 2012-06-06
PEOPLES HOSPITAL PEKING UNIV
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Problems solved by technology

Interferon-alpha (IFN-α) combined with ribavirin is currently the most effective treatment, but it requires subcutaneous injection of medication, the medication cycle is very long, the cost of treatment is expensive, and there are many adverse factors such as side effects of drugs; and interference HCV sustained virological response after antibiotic therapy is only about 50%

Method used

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  • Protein chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment
  • Protein chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment
  • Protein chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment

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Experimental program
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Effect test

Embodiment

[0040] 10ml of anticoagulated blood was taken from 12 patients with chronic hepatitis C before treatment with long-acting interferon plus ribavirin, and peripheral blood mononuclear cells were separated by Ficoll density gradient centrifugation. The separated mononuclear cells were equally divided into control group and experimental group. Group. The control group was cultured with DMEM medium for 48 hours, and the experimental group was treated with DMEM medium containing 100 U / ml IFNα for 48 hours to extract the protein of the above samples.

[0041] The protein chip was used to detect the change value of the relative expression level of ISG in the experimental group and the control group, and the total score of ISG was evaluated according to the above scoring standard, as shown in Table 2, in which:

[0042] The total ISG expression score of protein chip in 5 patients was 33-39 points (group 1), predicting that the probability of obtaining sustained virological response aft...

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Abstract

The invention relates to a protein chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment. The protein chip comprises protein fragments corresponding to 13 genes of ISG56, ISG20 and the like. Through the protein chip, in an early stage of chronic hepatitis C interferon treatment on patients or before the chronic hepatitis C interferon treatment on patients, interferon treatment long-term effects can be predicted. Therefore, the protein chip is conducive to optimization and selection of a chronic hepatitis C patient therapeutic schedule, reduces patient pain and treatment costs, and is convenient for detection and clinical application.

Description

【Technical field】 [0001] The invention relates to a protein chip, in particular to a chronic hepatitis C outcome prediction protein chip. 【Background technique】 [0002] Hepatitis C virus (HCV) infection is one of the most important viral infectious diseases at present. About 170 million people in the world are chronically infected with HCV, and chronic HCV infection may further develop into liver cirrhosis or even liver cancer. As there is currently no effective vaccine, there are still three to four million new HCV infections each year. Interferon-alpha (IFN-α) combined with ribavirin is currently the most effective treatment, but it requires subcutaneous injection of medication, the medication cycle is very long, the cost of treatment is expensive, and there are many adverse factors such as side effects of drugs; and interference The sustained virologic response to HCV after antibiotic therapy is only about 50%. Therefore, the discovery of factors that can predict the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 潘孝本魏来
Owner PEOPLES HOSPITAL PEKING UNIV
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