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Method for detecting seed vitality of cruciferae

A cruciferous and detection method technology, applied in germination equipment and other directions, can solve the problems of short detection life, cumbersome operation, long time consumption, etc., and achieve the effect of real-time online detection and fast analysis.

Inactive Publication Date: 2012-06-20
EAST CHINA UNIV OF TECH
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Problems solved by technology

Fluorescence method can be measured with only one UV fluorescence instrument without reagents, suitable for detecting seeds with short lifespan and large difference in vigor, and it is difficult to distinguish seeds with small difference in vigor
The above-mentioned methods, except the visual method, all destroy the seeds to a certain extent, and the tested seeds can no longer be used in production, and it takes a long time
The germination test method stipulated in the international standard procedure is relatively intuitive and has clear indicators, but usually an experiment takes 6-10 days, which is time-consuming and cumbersome to operate

Method used

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  • Method for detecting seed vitality of cruciferae
  • Method for detecting seed vitality of cruciferae
  • Method for detecting seed vitality of cruciferae

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Embodiment Construction

[0011] A method for detecting the vigor of cruciferous seeds is: first build a surface desorption chemical ionization source, the surface desorption chemical ionization source includes a discharge needle 4 and two stainless steel tee sleeves connected in series, and the discharge needle is interspersed between the two series Among the connected stainless steel tee bushings, two serially connected stainless steel tee bushings are separated by a partition, and the discharge needle 4 is coated with an insulating layer 1 except for both ends. One end is connected to a high-voltage power supply, and the other end is a At the discharge end, the outer layer of the discharge needle 4 is sleeved with a solvent quartz capillary 2 from the discharge end to the partition section. The front end of the solvent quartz capillary 2 communicates with the three-way sleeve near the high-voltage end, and the outlet of the three-way sleeve near the discharge end is connected to the discharge end. A ...

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Abstract

The invention relates to a method for detecting seed vitality of cruciferae. In the condition without processing a sample, the concentration of sinapine is used as a vitality marker, the surface desorption atmospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) is adopted, so that the content of the sinapine in the seed of the cruciferae is directly and quickly measured to judge the level of the vitality of the seed. According to the, the analysis speed is quick, the seed can be detected in batch without being damaged, the detected seed can also be used for agricultural production, and the quick and real-time on-line detection of quality of the seed of the cruciferae is realized.

Description

technical field [0001] The invention relates to a method for detecting the vigor of cruciferous seeds. Background technique [0002] Seed vigor is an important indicator to describe the quality of seeds. Seeds with high vigor have high germination rate and obvious growth advantage, which is of great significance to increase agricultural production. At present, the methods for detecting seed vigor include visual inspection, X-ray radiography, ethylene content method, conductivity method, staining method and fluorescence method. The visual method is to observe the plumpness, color, smell, thousand-grain weight, etc. of the seeds. Based on intuitive experience, there is no certain quantification, and there are shortcomings of large errors and unreliable results. X-ray radiography, using BaCl 2 Seeds are soaked in heavy metal salt solution, and the relationship between cell membrane activity and osmotic strength is used to develop the seeds. The image of seeds with high vitali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
Inventor 陈焕文姜翠翠崔莎莎贾滨张兴磊张华
Owner EAST CHINA UNIV OF TECH
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