Recombination ubiquitin ligase SH2-U-box fusion gene as well as expression vectors and applications thereof

Abstract

The invention discloses a recombination ubiquitin ligase SH2-U-box fusion gene as well as expression vectors and applications thereof. The recombination ubiquitin ligase SH2-U-box fusion gene is characterized in that an Src Homology 2 (SH2) structural domain gene of a growth factor receptor bound protein (Grb2) is connected with a ubiquitin-box (U-box) structural domain gene of a carboxy terminus of Hsc70 interacting protein (CHIP), and a concrete nucleotide sequence is shown as SEQ.ID.NO.1. The constructed SH2-U-box fusion gene can be cloned to different expression vectors, and thereby, the constructed SH2-U-box fusion gene enters cancer cells through different paths to perform actions, wherein the expression vectors comprise eukaryotic expression vectors or adenovirus expression vectors. The fusion gene is capable of knocking down carcinogenic related proteins BCR / Abl of host cancer cells after transfecting the cancer cells, thus, the cell proliferation capacity of the cancer cells is obviously reduced, and proliferation inhibition and invasion inhibition are shown on the cancer cells, thereby, an anti-cancer effect is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to gene recombination and its expression, in particular to a recombinant ubiquitin ligase SH2-U-box fusion gene and its expression vector and application. Background technique [0002] 1. Overview of CHIP [0003] CHIP (Carboxy terminus of Hsc70 Interacting Protein) is a kind of evolutionarily conserved and widely distributed cytoplasmic protein, which was originally considered as a co-chaperone. Since its C-terminus contains a U-box domain, it essentially belongs to a class of E3 ubiquitin ligases. CHIP plays an important role in protein quality control and stability regulation by promoting protein ubiquitination and degradation. In breast cancer tissue, the expression of CHIP is negatively correlated with tumor grade; studies have shown that CHIP can inhibit tumor proliferation and metastasis. It suggested that enhancing the effect of CHIP might be a new tumor treatment strategy. [...

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Problems solved by technology

However, there is no report on the use of CHIP ubiquitin ligase activity to construct recombinant ubiquitin ligases for gene therapy research.

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